Essential Zebrafish Methods: Genetics and Genomics

1. Haploid Screens and Gamma-Ray Mutagenesis (Charline Walker) 2. Positional Cloning of Mutated Zebrafish Genes (Will Talbot) 3. The Zebrafish Genome (John Postlethwait) 4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish (Adam Amsterdam) 5. Genetic Screens for Maternal-Effect Mutations (Francisco Pelegri) 6. Behavior Screening Assays in Zebrafish (Herwig Baier) 7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish (Cecelia B. Moens) 8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element (Koichi Kawakami)9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish (Charles Lee) 10. The Zon Laboratory Guide to Positional Cloning in Zebrafish (Leonard I. Zon) 11. Sleeping Beauty Transposon for Efficient Gene Delivery (Stephen C. Ecker) 12. Transgene Manipulation in Zebrafish by Using Recombinases (Gary W. Stuart) 13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI (Joachim Wittbrodt) 14. Cloning Zebrafish by Nuclear Transfer (Shuo Lin) 15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening (Christine Thisse) 16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish (Bill Trevarrow) 17. Common Diseases of Laboratory Zebrafish (Jennifer L. Matthews) 18. Zebrafish Sperm Cryopreservation (Thomas Look)

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms. Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA