Essentials of Stem Cell Biology

FOREWORD

Why Stem Cell Research?
A New Path ?{ Induced Pluripotent Stem Cells
Embryonic Stem Cells Versus Adult Stem Cells
'Stemness': Definitions, Criteria and Standards

PART ONE
INTRODUCTION TO STEM CELLS
1. Present Perspective and Future Challenges
2. Embryonic Stem Cells in Perspective
3. The Development of Epithelial Stem Cell Concepts
4. The Advent of Direct Reprogramming
5. Clinical Translation of Stem Cells

PART TWO
BASIC BIOLOGY/MECHANISMS
6. Molecular Basis of Pluripotency
7. Stem Cell Niches
8. Mechanisms of Stem Cell Self-Renewal
9. Cell Cycle Regulators in Stem Cells
10. Epigenetic Mechanisms of Cellular Memory
11. Cell Fusion and the Differentiated State
12. How Cells Change Their Phenotype

PART THREE
TISSUE AND ORGAN DEVELOPMENT
13. Differentiation in Early Development
14. Primordial Germ Cells in Mouse and Human
15. Stem Cells in Extraembryonic Lineages
16. Amniotic Fluid Derived Pluripotent Cells
17. Cord Blood Stem Cells
18. Neurogenesis in the Vertebrate Embryo
19. The Nervous System
20. Neuronal Progenitors in the Adult Brain
21. Sensory Epithelium of the Eye and Ear
22. Epithelial Skin Stem Cells
23. The Ontogeny of the Hematopoietic System
24. Hematopoietic Stem Cells
25. Red Blood Cells
26. Cell Differentiation in the Skeleton
27. Vascular Progenitor Cells
28. Multipotent Adult Progenitor Cells
29. Mesenchymal Stem Cells
30. Skeletal Muscle Stem Cells
31. Stem Cells and the Regenerating Heart
32. Potential of ES Cell Differentiation Culture for Vascular Biology
33. Cell Lineages and Stem Cells in the Kidney
34. Adult Liver Stem Cells
35. Pancreatic Stem Cells
36. Stem Cells in the Gastrointestinal Tract
37. Stem Cells in the Lung

PART FOUR
METHODS
38. Generation of Induced Pluripotent Stem Cells
39. Characteristics and Characterization of Pluripotent Stem Cells
40. Isolation and Maintenance of Murine Embryonic Stem Cells
41. Isolation, Characterization, and Maintenance of Primate

Robert Lanza, M.D. is currently Chief Scientific Officer at Advanced Cell Technology, and Adjunct Professor of Surgical Sciences at Wake Forest University School of Medicine. He has several hundred scientific publications and patents, and over 30 books, including Principles of Tissue Engineering (1st through 4th Editions), Methods of Tissue Engineering, Principles of Cloning (1st and 2nd Editions), Essentials of Stem Cell Biology (1st and 2nd Editions), XENO, Yearbook of Cell & Tissue Transplantation, One World: The Health & Survival of the Human Species in the 21st Century (as editor, with forewords by C. Everett Koop and former President Jimmy Carter), and Medical Science & the Advancement of World Health. Dr. Lanza received his B.A. and M.D. degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. He is a former Fulbright Scholar, and studied as a student in the laboratory of Richard Hynes (MIT), Jonas Salk (The Salk Institute), and Nobel laureates Gerald Edelman (Rockefeller University) and Rodney Porter (Oxford University). He also worked closely (and coauthored a series of papers) with the late Harvard psychologist B.F. Skinner and heart transplant pioneer Christiaan Barnard. Dr. Lanza's current area of research focuses on the use of stem cells in regenerative medicine.