Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators.- Tandem Affinity Purification Approach Coupled To Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-binding Proteins.- Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos.- Systematic Discovery Of Chromatin-bound Protein Complexes from ChIP-seq Datasets.- Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) Protocol for Zebrafish Embryos.- Establishment of time- and cell-specific RNAi in Caenorhabditis elegans.- Cell-penetrating Peptide-mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing.- Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.- eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response.- High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA using 4-thiouridine.- Accurate Detection of Differential Expression and Splicing using Low-level Features.- Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.- Determining if an mRNA is a Substrate of Nonsense-mediated mRNA Decay in Saccharomyces cerevisiae.- Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract.-  Unbiased Interrogation of 3D Genome Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq).- Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells.- In vitro assay to Study Histone Ubiquitination during Transcriptional Regulation.- Determination of Alternate Splicing Events using Transcriptome