Extracellular Vesicles

Reliable Method for Exosome Purification

            Richard Lobb and Andreas Möller

 

10. Purification Protocols for Extracellular Vesicles             Rebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill

 

Part III: Characterization of Extracellular Vesicles

 

11. Characterization of Extracellular Vesicles by Surface Plasmon Resonance

            Hyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro

 

12. Extracellular Vesicle Isolation and Analysis by Western Blotting             Emma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church

 

13. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis

            Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic

 

14. Characterization of Extracellular Vesicles by Flow Cytometry

            Virginia Camacho, Vasilis Toxavidis, and John C. Tigges

  15. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)

            Tao Huang and Jiang He

 

16. Multi-Surface Antigen Staining of Larger Extracellular Vesicles

            Veronika Lukacs-Kornek, Henrike Julich, Sabine Katharina Urban, and Miroslaw Kornek

 

17. Microcapillary Chip-Based Extracellular Vesicle Profiling System

            Takanori Akagi and Takanori Ichiki  

Part IV: Imaging of Extracellular Vesicles

 

18. Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy

            Petr Cizmar and Yuana Yuana

 

19. Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy

            Dmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci

  Part V: Tracking of Extracellular Vesicles

 

20. In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase   

            Yuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura

 

21. Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters

            Anthony Yan-Tang Wu and Charles Pin-Kuang Lai

 

Part VI: Downstream Extracellular Vesicle Applications Genomics and Proteomics

 

22. Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples

            Davide Zocco and Natasa Zarovni

 

23. Extracellular Vesicle miRNA Detection Using Molecular Beacons

            Won Jong Rhee and Seunga Jeong

 

24. Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling

            Simion Kreimer and Alexander R. Ivanov

 

25. An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles

            Stephanie N. Hurwitz and David G. Meckes, Jr.

 

Part VII: Isolation of Extracellular Vesicles from Biofluids

 

26. Isolation of Extracellular Vesicles from Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits

            Shlomit Kenigsberg, Clifford C. Librach, and Juliano C. da Silveira

 

27. Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation

            Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, and Kiyotaka Shiba

 

28. Isolation of Extracellular Vesicles from Breast Milk

            Xin Wang

  29. An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis

            Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, and ShuQi Wang

 

Part VIII: Extracellular Vesicles Isolated from Cell Culture, Parasites, and Stem Cells

 

30. Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles

            Chris Wang, Austin Wang, Fang Wei, David T.W. Wong, and Michael Tu  

31. Production and Characterization of Extracellular Vesicles in Malaria

            Smart Mbagwu, Michael Walch, Luis Filgueira, and Pierre-Yves Mantel

 

32. Isolation of Extracellular Vesicles from Stem Cells

            Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, and Yaoliang Tang

 

Part IX: Extracellular Vesicles in Mouse Models

  33. The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model

            Noemí García-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, and Angel Ayuso-Sacido

 

Part X: Therapeutic Applications of Extracellular Vesicles

 

34. Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine

            Gareth R. Willis, Stella Kourembanas, and S. Alex Mitsialis

  35. Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles

            Jing Liu, Jingwei Ma, Ke Tang, and Bo Huang