Food-Borne Pathogens
Methods and Protocols
(Sprache: Englisch)
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including...
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Klappentext zu „Food-Borne Pathogens “
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Inhaltsverzeichnis zu „Food-Borne Pathogens “
The Bacteria.- Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources.- Detection and Purification of Bacillus cereus Enterotoxins.- Campylobacter.- Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces.- Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex.- PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis.- Plesiomonas shigelloides.- Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies.- Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus.- Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples.- HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes.- The Viruses.- Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR.- Detection of Enteroviruses.- Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification.- The Parasites.- Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis.- Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish.- Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.).- Parallel Studies to the Analysis of Food-Borne Pathogens.- Approaches to Developing Quantitative Risk Assessment Models.- A Review of Surveillance Networks of Food-Borne Diseases.
Bibliographische Angaben
- 2010, XII, 268 Seiten, Maße: 15,2 x 22,9 cm, Kartoniert (TB), Englisch
- Herausgegeben: Catherine Adley
- Verlag: Springer, Berlin
- ISBN-10: 1617375853
- ISBN-13: 9781617375859
Sprache:
Englisch
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