Fundamentals of Fluorescence Microscopy
Exploring Life with Light
(Sprache: Englisch)
Guiding readers from fundamental concepts to the most advanced fluorescence imaging techniques, this thorough introduction to the topic is ideal for students in a variety of disciplines and includes a detailed explanation of molecular orbital theory.
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Guiding readers from fundamental concepts to the most advanced fluorescence imaging techniques, this thorough introduction to the topic is ideal for students in a variety of disciplines and includes a detailed explanation of molecular orbital theory.
Klappentext zu „Fundamentals of Fluorescence Microscopy “
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Inhaltsverzeichnis zu „Fundamentals of Fluorescence Microscopy “
Preface.- Acknowledgements.- Dedication.- Ray Optics, Wave Optics and Imaging System Designing.- Basics of Electromagnetic Theory for Fluorescence Microscopy.- Electric Field Effects in Optical Microscopy Systems.- Quantum Description of Radiation Field and Optical Microscopy.- Molecular Physics of Fluorescence Markers.- Basics of Fluorescence and Photophysics.- General Fluorescence Imaging Techniques.- Multiphoton Fluorescence Microscopy.- Super Resolution Fluorescence Microscopy.- Image Reconstruction Methodologies for Fluorescence Microscopy.- Future Prospective of Fluorescence Microscopy.- Appendix I.- Appendix II.- Appendix III.
Bibliographische Angaben
- Autoren: Partha Pratim Mondal , Alberto Diaspro
- 2016, Softcover reprint of the original 1st ed. 2014., 218 Seiten, Kartoniert (TB), Englisch
- Verlag: Springer Netherlands
- ISBN-10: 940177966X
- ISBN-13: 9789401779661
Sprache:
Englisch
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