Gene Quantification

Key Issues, Challenges and Future Opportunities in Gene Quantification.- I: Methods/Technology Issues.- A. Gene Quantitation Based on PCR Amplification.- Present and Future Detection Formats for PCR Quantitation of Nucleic Acids.- Determination of Target Copy Number of Quantitative Standards Used in PCR Based Diagnostic Assays.- Quantification of Specific Nucleic Acids, Regulated RNA Processing and Genomic Polymorphisms Using Reversed-Phase HPLC.- Capillary Electrophoresis for Quantitative Genetic Analysis.- Quantitative PCR Technology.- Statistical Estimations of PCR Amplification Rates.- Fluorescence Monitoring of Rapid Cycle PCR for Quantification.- Kinetic Elisa-PCR: A Versatile Quantitative PCR Method.- B. Gene Quantitation Based on Other Target Amplification Systems.- Quantitation of RNA by NASBA(TM): Applications and Issues for HIV-1 and AIDS.- Application of Transcription-Mediated Amplification to Quantification of Gene Sequences.- C. Gene Quantitation Based on Signal Amplification.- Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Design and Performance.- Hybrid Capture(TM) - A Sensitive Signal Amplified Test for the Detection and Quantitation of Human Viral and Bacterial Pathogens.- II: Applications.- Quantification of Gene Expression by Competitive RT-PCR: The hCG?/LH? Gene Cluster.- Quantitative Detection of Mycoplasma DNA Using Competitive PCR.- The Detection and Quantification of bcr-abl in Chronic Myeloid Leukemia Following Marrow Transplantation.- Competitive RT-PCR Analysis of Brain Gene Expression During Inflammation and Disease.- Development and Application of Real-Time Quantitative PCR.- Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Research and Clinical Applications.- Quantification of PlasmidDNA Expression in Vivo.