Glycomics: Methods and Protocols
(Sprache: Englisch)
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists...
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Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Inhaltsverzeichnis zu „Glycomics: Methods and Protocols “
Section I: Glycan AnalysisSubsection A: Released Glycans1. Analysis of N- and O-Linked Glycans from Glycoproteins Using Maldi-TOF Mass SpectrometryWilly Morelle, Valegh Faid, Frédéric Chirat, and Jean-Claude Michalski2. Infrared Multiphoton Dissociation (IRMPD) Mass Spectrometry for Structural Elucidation of OligosaccharidesBensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla3. Mass Spectrometry of N-linked GlycansParastoo Azadi and Christian Heiss4. Solid-phase Permethylation for Glycomic AnalysisYehia Mechref, Pilsoo Kang, and Milos V. Novotny5. Method for Investigation of Oligosaccharides Using Phenylhydrazine DerivatizationErika Lattová and Hélène Perreault6. 2-Dimensional HPLC Separation with Reverse Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools after Labeling with 2-AminobenzamideManfred Wuhrer, Carolien A. M. Koeleman, and André M. Deelder7. Capillary Lectin-Affinity Electrophoresis for Glycan AnalysisKazuaki Kakehi and Mitsuhiro Kinoshita8. Analysis of Methylated O-Glycan Alditols by CAD-ESI Mass SpectrometryFranz-Georg Hanisch and Stefan Müller 9. High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan ProfilingHasse Karlsson, Jessica M. Holmén Larsson, Kristina A. Thomsson, Iris Härd, Malin Bäckström, and Gunnar C. Hansson10. Collision Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of GlycansBensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla11. The Structural Elucidation of GlycosaminoglycansVikas Prabhakar, Ishan Capila, and Ram Sasisekharan12. Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescence and Mass Tags for HPLC and MS SeparationsMark Skidmore, Abdel Atrih, Ed Yates, and Jeremy E. Turnbull13. Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel ElectrophoresisRuby P. Estrella, John M. Whitelock, Rebecca H. Roubin, Nicolle H. Packer, and Niclas G. Karlsson
... mehr
Subsection B: Glycopeptides14. Enrichment Strategies for GlycopeptidesShigeyasu Ito, Ko Hayama, and Jun Hirabayashi15. Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass FingerprintingNicole Wilson, Raina Simpson, and Catherine Cooper-Liddell16. Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim, and Tillman Gerngross17. N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MSUrs Lewandrowski and Albert Sickmann18. LC/MSn For Glycoprotein Analysis: N-Linked Glycosylation Analysis And Peptide Sequencing Of GlycopeptidesNana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi Subsection C: O-GlcNAcomics19. Detecting the "O-GlcNAc-ome"; Detection, Purification, and Analysis of O-GlcNAc Modified ProteinsNatasha E. Zachara Subsection D: Glycobioinformatic Tools20. Preparation of a Glycan Library Using a Variety of GlycosyltrasferasesHiromi Ito, Akihiko Kameyama, Takashi Sato, and Hisashi Narimatsu21. Data Mining the PDB for Glyco-Related DataThomas Lütteke and Claus-W. von der Lieth Section II: Carbohydrate-Protein Interactions22. Saccharide Microarrays for High-Throughput Interrogation of Glycan-Protein Binding InteractionsAndrew Powell, Zheng-liang Zhi, and Jeremy E. Turnbull23. Glycosaminoglycan Characterization Methodologies: Probing Biomolecular InteractionsVikas Prabhakar, Ishan Capila, and Ram Sasisekharan24. Development and Characterization of Antibodies to Carbohydrate AntigensJamie Heimburg-Molinaro and Kate Rittenhouse-Olson25. Inhibition of Glycosyltransferase Activities as the Basis for Drug DevelopmentJohn Schutzbach and Inka Brockhausen26. Saturation Transfer Difference (STD) NMR Spectroscopy as a Technique to Investigate Protein-Carbohydrate Interactions in SolutionThomas Haselhorst, Anne-Christin Lamerz, and Mark von Izstein
... weniger
Bibliographische Angaben
- 2009, 400 Seiten, Maße: 19,7 x 27 cm, Gebunden, Englisch
- Herausgegeben: Nicolle H. Packer, Niclas G. Karlsson
- Verlag: Springer-Verlag GmbH
- ISBN-10: 1588297748
- ISBN-13: 9781588297747
Sprache:
Englisch
Rezension zu „Glycomics: Methods and Protocols “
From the reviews:"This book discusses methods of a comparatively new direction in science, which has been named glycomics. ... The book consists two parts with 26 chapters prepared by well-known experts from many countries. ... I confirm the book fulfills the goal and I consider this book as an excellent practical guide to glycomics methods. Each method is described in great detail, and the whole book is excellent manual for many researchers in biochemistry, molecular biology, immunochemistry, biotechnology, and medicine." (G. Wiederschain, Biochemistry, Vol. 75 (4), 2010)
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