Hedgehog Signaling

Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells.- Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-Coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay.- Analysis of Protein Cholesterylation by Biorthogonal Labeling.- An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched.- Imaging of the Ciliary Cholesterol Underling the Sonic Hedgehog Signal Transduction.- Studying Hedgehog Signaling during Mouse Neural Tube Development.- Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling.- The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.- Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release.- Expression, Purification, and Structure Determination of Human PTCH1-HH-N Complexes.- Characterization of Smoothened Phosphorylation and Activation.- Cell-Based Assays for Smoothened Ubiquitination and Sumoylation.- Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody.- Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems.- Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors.- Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG.- NanoBRET and NanoBiT/BRET-Based Ligand Binding Assays Permit Quantitative Assessment of Small Molecule Ligand Binding to Smoothened.- Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein.- Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases.- A Method for Orthotopic Transplantation of Lung Cancer in Mice.