Heterologous Expression of Wheat Proteins in Yeast

The expression of proteins from different host organisms is of big interest in the modern biotechnology. Not only for commercial or medical purposes, but also for the characterization of foreign proteins the heterologous expression plays a significant role.How can a foreign protein be expressed in a pro- or eukaryotic host? Which host is suitable for which kind of protein? How can the specific protein yield be increased by varying process parameter?The author Dr. Falk Matthäus gives introductorily an overview of different host systems for the heterologous expression of proteins. The high cell density fermentation and the applicability of different plasmid systems are discussed in the introduction. The heterologous expression of the putatively celiac disease triggering proteins of the wheat endosperm is the central point of this book. The optimization of fermentation parameter for higher biomass and protein yield are discussed. Furthermore, the adaptation of the classical protein purification to the changed host system as well as the establishment of an aqueous two phase system as alternativ protein purification are discussed.