Immunological Tolerance

Isolation and Characterisation of Human Adipose-derived Stem Cells.- Isolation of Murine Hematopoietic Stem Cells.- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells.- Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting .- Identification and isolation of Regulatory B cells in Mouse and Human.- An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis.- Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells.- Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade.- In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells.- Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays.- Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments.- Immune tolerance and rejection in organ transplantation.- Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation.- Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood.- Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental.- Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle.