Introduction to Fluorescence Sensing

Chapter 1. Introduction



Chapter 2. Basic principles



2.1. Overview of strategies in molecular sensing

2.2. Labeled targets in fluorescence assays

2.3. Competitor displacement assays

2.4. Sandwich assays

2.5. Catalytic biosensors

2.6. Direct reagent-independent sensing

Sensing and thinking: How to make the best sensor? Comparison of basic principles



Chapter 3. Theoretical aspects



3.1. Parameters that need to be optimized in every sensor

3.2. Determination of binding constants

3.3. Modeling the ligand binding isotherm

3.4. Kinetics of target binding

3.5. Formats for fluorescence detection

Sensing and thinking: How to provide the quantitative measure of target binding?



Chapter 4. Fluorescence detection techniques



4.1. Fluorescence fundamentals

4.2. Intensity-based sensing

4.3. Anisotropy-based sensing and polarization assays

4.4. Lifetime-based fluorescence response

4.5. Excimer and exciplex formation

4.6. Förster resonance energy transfer (FRET)

4.7. Wavelength-shift sensing

4.8. Two-band wavelength-ratiometric sensing with a single dye

Sensing and thinking: The optimal choice of fluorescence detection technique



Chapter 5. Molecular-size fluorescence emitters



5.1. Fluorophores and their characteristics

5.2. Organic dyes as labels and tags

5.3. Organic dyes as fluorescence reporters

5.4. Visible fluorescent proteins

5.5. Luminescent metal complexes

5.6. Few-atom clusters of noble metals

Sensing and thinking: Which molecular reporter to choose for particular needs?



Chapter 6. Nanoscale fluorescence emitters



6.1.