Laboratory Experiments in Microbiology

Introduction. I. MICROSCOPY. 1. Use and Care of the Microscope. 2. Examination of Living Microorganisms. II. STAINING METHODS. 3. Preparation of Smears and Simple Staining. 4. Negative Staining. 5. Gram Staining. 6. Acid-fast Staining. 7. Structural Stains (Endospore, Capsule, Flagella). 8. Morphologic Unknown. III. CULTIVATION OF BACTERIA. 9. Microbes in the Environment. 10. Transfer of Bacteria: Aseptic Techniques. 11. Isolation of Bacteria by Dilution Technique. 12. Special Media for Isolating Bacteria. IV. MICROBIAL METABOLISM. 13. Carbohydrate Catabolism. 14. Fermentation. 15. Protein Catabolism, Part 1. 16. Protein Catabolism, Part 2. 17. Respiration. 18. Rapid Identification Methods. V. MICROBIAL GROWTH. 19. Oxygen and the Growth of Bacteria. 20. Determination of a Bacterial Growth Curve: The Role of Temperature. 21. Biofilms. VI. CONTROL OF MICROBIAL GROWTH. 22. Physical Methods of Control: Heat. 23. Physical Methods of Control: Ultraviolet Radiation. 24. Chemical Methods of Control: Disinfectants and Antiseptics. 25. Chemical Methods of Control: Antimicrobial Drugs. 26. Effectiveness of Hand Scrubbing. VII. MICROBIAL GENETICS. 27. Regulation of Gene Expression. 28. Isolation of Bacterial Mutants. 29. Transformation of Bacteria. 30. DNA Fingerprinting. 31. Genetic Engineering. 32. Ames Test for Detecting Possible Chemical Carcinogens. VIII. THE MICROBIAL WORLD. 33. Unknown Identification and Bergey's Manual. 34. Fungi: Yeasts. 35. Fungi: Molds. 36. Phototrophs: Algae and Cyanobacteria. 37. Protozoa. IX. VIRUSES. 38. Isolation and Titration of Bacteriophages. 39. Plant Viruses. X. INTERACTION OF MICROBE AND HOST. 40. Epidemiology. 41. Koch's Postulate. XI. IMMUNOLOGY. 42. Nonspecific Resistance. 43. Blood Group Determination: Slide Agglutination. 44. Agglutination Reactions: Microtiter Agglutination. 45. ELISA Technique. XII. MICROORGANISMS AND DISEASE. 46. Bacteria of the Skin. 47. Bacteria of the Respiratory Tract. 48. Bacteria of the Mouth. 49. Bacteria of