Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
(Sprache: Englisch)
This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles. Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools guides readers through methods...
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This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles. Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Lentiviral Vectors andExosomes as Gene and Protein Delivery Tools aims to ensure successfulresults in the further study of this vital field.
Inhaltsverzeichnis zu „Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools “
Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown.- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase.- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions.- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G.- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors.- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors.- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.- The LAM-PCR Method to Sequence LV Integration Sites.- Conditional Rnai using the Lentiviral GLTR System.- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies.- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line.- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease.- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery.- Biogenesis and Functions of Exosomes and Extracellular Vesicles.- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes.- Cardiac Myocyte Exosome Isolation.- Incorporation of Heterologous Proteins in Engineered Exosomes.- Exosome-Mediated Targeted Delivery of Mirnas.Bibliographische Angaben
- 2018, Softcover reprint of the original 1st ed. 2016, XI, 276 Seiten, 33 farbige Abbildungen, Maße: 17,8 x 25,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Maurizio Federico
- Verlag: Springer, Berlin
- ISBN-10: 1493981293
- ISBN-13: 9781493981298
Sprache:
Englisch
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