Liposome Methods and Protocols
(Sprache: Englisch)
In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in...
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In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.
Inhaltsverzeichnis zu „Liposome Methods and Protocols “
Part I. Physical Properties- Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids
- Subroto Chatterjee and Dipak K. Banerjee
- Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems
- Massimo Masserini, Paola Palestini, Marina Pitto, Vanna Chigorno, and Sandro Sonnino
Part II. Liposome Fusion/Modulation
- Peptide-Induced Fusion of Liposomes
- Eve-Isabelle Pécheur and Dick Hoekstra
- Liposomes: Applications in Protein-Lipid Interaction Studies,
- Sujoy Ghosh and Robert Bell
- Lipids in Viral Fusion
- Anu Puri, Maite Paternostre, and Robert Blumenthal
Part III. Application of Liposomes
- Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
- Shimon Gatt, Tama Dinur, and Arie Dagan
- Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays
- Manju Basu and Subhash Basu
- Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
- Roger K. Bretthauer and Dennis W. Welsh
- Therapeutic Uses of Antioxidant Liposomes
- William L. Stone, Shyamali Mukherjee, Milton Smith, and Salil K. Das
- Targeted Gene Delivery by Virosomes
- Debi P. Sarkar, Komal Ramani, and Sandeep K. Tyagi
- Liposomes Containing Ligands: Binding Specificity to Selectins
- Sriram Neelamegham and Khushi L. Matta
- Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
- P. R. Satish and A. Surolia
- Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies
- Reiji Kannagi
- Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides
- Takao Taki and Naoto Oku
- Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins
- Francis J. Castellino and Eric H. Ellison
- Index
Bibliographische Angaben
- 2002, 252 Seiten, Maße: 23,5 cm, Gebunden, Englisch
- Herausgegeben von Basu, Subhash C.; Basu, Manju
- Verlag: Humana Press
- ISBN-10: 0896038459
- ISBN-13: 9780896038455
Sprache:
Englisch
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