Mass Spectrometry for Microbial Proteomics
(Sprache: Englisch)
New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is...
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Klappentext zu „Mass Spectrometry for Microbial Proteomics “
New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens.This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject -Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.
Inhaltsverzeichnis zu „Mass Spectrometry for Microbial Proteomics “
PrefaceList of contributors Microbial Characterisation; the Transition from Conventional Methods to Proteomics.
1) CHANGING CONCEPTS IN THE CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY
Haroun Shah et al
2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES (THE CHANGE FROM TARGETED GENES TO PROTEINS)
Radhey Gupta
2: PROTEOMICS TOOLS AND BIOMARKER DISCOVERY.
3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT LINKS TO GENOMICS
Raju Misra.
4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN MICROORGANISMS
Ming Fang
Proteomic Strategies for Protein Identification
1. Bottom-up Proteomics
2. Top-down Proteomics
Multidimensional Protein Identification
Mass Spectrometry Based Targeted Protein Quantification and Biomarker Discovery
Selected Reaction Monitoring
Conclusions
5) MALDI MASS SPECTROMETRY IMAGING, A NEW FRONTIER IN BIOSTRUCTURAL TECHNIQUES: APPLICATIONS IN BIOMEDICINE Simona Francese and Malcolm R. Clench
3: PROTEIN SAMPLES PREPARATION TECHNIQUES
CONVENTIONAL APPROACHES FOR SAMPLE PREPARATION FOR LIQUID
CHROMATOGRAPHY AND TWO-DIMENSIONAL GEL ELECTROPHORESIS
Vesela Encheva and Robert Parker
7) ISOLATION AND PREPARATION OF SPORE PROTEINS AND SUBSEQUENT CHARACTERISATION BY ELECTROPHORESIS AND MASS SPECTROMETRY Nicola Thorne, Saheer Gharbia and Haroun Shah
8) CHARACTERIZATION OF BACTERIAL MEMBRANE PROTEINS USING A NOVEL COMBINATION OF A LIPID BASED PROTEIN IMMOBILIZATION TECHNIQUE WITH MASS SPECTROMETRY
Roger Karlsson, Darren Chooneea, Elisabet Carlsohn, Vesela Encheva and Haroun Shah
9) Wider Protein Detection from Biological Extracts by the Reduction of Dynamic Concentration Range.
Luc Guerrier, Egisto Boschetti and Piergiorgi Roghetti
10) 3D-gel electrophoresis - a new development in protein
... mehr
analysis.
Robert Ventzki and Josef Stegemann
SECTION 4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION.
11) Microbial Disease Biomarkers using ProteinChip Arrays
Shea Hamilton, Michael Levin, J. Simon Kroll, Paul R. Langford
12) MALDI-TOF MS and microbial identification: years of experimental
development to an established protocol.
Wibke Kallow, Marcel Erhard,
Haroun N. Shah, Emmanuel Raptakis, Martin Welker.
5: Targeted Molecules and Analysis of Specific Microorganisms.
13) Whole Cell MALDI Mass Spectrometry for the Rapid Characterisation of
Bacteria; A Survey of Applications to Major Phyletic Lines in Microbial
Kingdom.
Ben van Baar
Bacillus spp.
Staphylococcus spp.
Streptococcus spp.
Mycobacterium spp.
Other Gram-positive bacteria
Escherichia coli
Gram-negative food- and waterborne pathogen proteobacteria, other than E. Coli
Typical sexually transmitted pathogens: Neisseria spp. and Haemophilus spp.
Gram-negative biothreat agent bacteria
Other Gram-negative bacteria
Pathogenic Cyanobacteria
Strategies for the identification of biomarkers in whole cell MALDI MS spectra
Protein database consideration
On-target treatment and analysis
Off-target' Analysis and correlation with proteomics studies
General consideration of biomarker identification strategies
Conclusions and outlook
14) The power of Gel-based proteomics to understand
physiology in Bacillus subtilis
&nb
Robert Ventzki and Josef Stegemann
SECTION 4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION.
11) Microbial Disease Biomarkers using ProteinChip Arrays
Shea Hamilton, Michael Levin, J. Simon Kroll, Paul R. Langford
12) MALDI-TOF MS and microbial identification: years of experimental
development to an established protocol.
Wibke Kallow, Marcel Erhard,
Haroun N. Shah, Emmanuel Raptakis, Martin Welker.
5: Targeted Molecules and Analysis of Specific Microorganisms.
13) Whole Cell MALDI Mass Spectrometry for the Rapid Characterisation of
Bacteria; A Survey of Applications to Major Phyletic Lines in Microbial
Kingdom.
Ben van Baar
Bacillus spp.
Staphylococcus spp.
Streptococcus spp.
Mycobacterium spp.
Other Gram-positive bacteria
Escherichia coli
Gram-negative food- and waterborne pathogen proteobacteria, other than E. Coli
Typical sexually transmitted pathogens: Neisseria spp. and Haemophilus spp.
Gram-negative biothreat agent bacteria
Other Gram-negative bacteria
Pathogenic Cyanobacteria
Strategies for the identification of biomarkers in whole cell MALDI MS spectra
Protein database consideration
On-target treatment and analysis
Off-target' Analysis and correlation with proteomics studies
General consideration of biomarker identification strategies
Conclusions and outlook
14) The power of Gel-based proteomics to understand
physiology in Bacillus subtilis
&nb
... weniger
Bibliographische Angaben
- 2010, 1. Auflage, 536 Seiten, Maße: 17,6 x 25,1 cm, Gebunden, Englisch
- Herausgegeben: Haroun N. Shah, Saheer E. Gharbia
- Verlag: Wiley & Sons
- ISBN-10: 0470681993
- ISBN-13: 9780470681992
- Erscheinungsdatum: 29.09.2010
Sprache:
Englisch
Rezension zu „Mass Spectrometry for Microbial Proteomics “
"Summary Mass Spectrometry of Microbial Proteomics provides an authoritative guide to the expanding field of microbial proteomics." ( Anal Bioanal Chem , 2011)
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"Summary Mass Spectrometry of Microbial Proteomicsprovides an authoritative guide to the expanding field of microbialproteomics." ( Anal Bioanal Chem , 2011)Kommentar zu "Mass Spectrometry for Microbial Proteomics"
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