Membrane Proteins Production for Structural Analysis
(Sprache: Englisch)
This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled...
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This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.
Inhaltsverzeichnis zu „Membrane Proteins Production for Structural Analysis “
.- 1 Membrane Proteins Production for Structural Analysis .- Isabelle Mus-Veteau, Pascal Demange, Francesca Zito
.- 2 Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies
.- Davide Proverbio, Erik Henrich, Erika Orbán, Volker Dötsch and Frank Bernhard
.- 3 Bacterial Expression and Stabilization of GPCRs
.- Jean Louis Banères
.- 4 Membrane Protein Production in Escherichia Coli: Overview and Protocols
.- Georges Hattab, Annabelle Y. T. Suisse, Oana Ilioaia, Marina Casiraghi, Manuela Dezi, Xavier Warnet, Dror Warschawski, Karine Moncoq, Manuela Zoonens and Bruno Miroux
.- 5 Lactococcus Lactis, Recent Developments in Functional Expression of Membrane Proteins
.- Sana Bakari, François André, Daphné Seigneurin-Berny, Marcel Delaforge, Norbert Rolland and Annie Frelet-Barrand
.- 6 Overexpression of Membrane Proteins in Saccharomyces Cerevisiae for Structural and Functional Studies: a Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid "Flippase" Complex Drs2p/Cdc50p
.- Cédric Montigny, Hassina Azouaoui, Aurore Jacquot, Marc le Maire, Christine Jaxel, Philippe Champeil and Guillaume Lenoir
.- 7 Amphipols: a General Introduction and Some Protocols
.- Manuela Zoonens, Francesca Zito, Karen L. Martinez and Jean-Luc Popot
.- 8 New Amphiphiles to Handle Membrane Proteins: "Ménage à Trois" between Chemistry, Physical-Chemistry and Biochemistry
.- Grégory Durand, Maher Abla, Christine Ebel and Cécile Breyton
.- 9 Building Model Membranes with Lipids and Proteins: Dangers and Challenges
.- James Sturgis
.- 10 Analytical Ultracentrifugation and Size Exclusion Chromatography Coupled to Light Scattering for the Characterization of Membrane Proteins in Solution
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.- Aline Le Roy, Cécile Breyton and Christine Ebel
.- 11 Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins
.- Andrii Ishchenko, Enrique Abola and Vadim Cherezov
.- 12 Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes or Intact Cells: the Hitchhiker's Guide to the Membrane Protein Study by NMR
.- Laurent J. Catoire, Xavier L. Warnet And Dror E. Warschawski
.- 13 Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations and Free Energy Calculations of Membrane Proteins
.- Jérôme Hénin, Marc Baaden, Antoine Taly
.- 14 Structural Studies of TSPO, a Mitochondrial Membrane Protein
.- Jean-Jacques Lacapere, Soria Iatmanen-Harbi, Lucile Senicourt, Olivier Lequin, Piotr Tekely, Rudra N. Purusottam, Petra Hellwig, Sebastien Kriege, Stephanie Ravaud, Céline Juillan-Binard, Eva Pebay Peyroula, Vassilios Papadopoulos
.- Aline Le Roy, Cécile Breyton and Christine Ebel
.- 11 Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins
.- Andrii Ishchenko, Enrique Abola and Vadim Cherezov
.- 12 Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes or Intact Cells: the Hitchhiker's Guide to the Membrane Protein Study by NMR
.- Laurent J. Catoire, Xavier L. Warnet And Dror E. Warschawski
.- 13 Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations and Free Energy Calculations of Membrane Proteins
.- Jérôme Hénin, Marc Baaden, Antoine Taly
.- 14 Structural Studies of TSPO, a Mitochondrial Membrane Protein
.- Jean-Jacques Lacapere, Soria Iatmanen-Harbi, Lucile Senicourt, Olivier Lequin, Piotr Tekely, Rudra N. Purusottam, Petra Hellwig, Sebastien Kriege, Stephanie Ravaud, Céline Juillan-Binard, Eva Pebay Peyroula, Vassilios Papadopoulos
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Autoren-Porträt
Dr. Isabelle Mus-Veteau earned her PhD in Microbiology and Cell Biology at Centre National de la Recherche Scientifique (CNRS) in Marseille, France with a thesis entitled, "Development of models to study structure-function relationship of multihemic cytochrome and its oxydoreduction partners". She then earned her HDR - in Europe, the highest academic qualification a scholar can achieve through his/her own pursuit - from Nice University, in France. She is currently a member of the faculty at the Institute of Molecular and Cellular Pharmacology (IPMC), Joint Research Unit- Centre National de la Recherche Scientifique (UMR-CNRS) in Valbonne, France, where she works closely with PhD students and is also project leader for research on hedgehog pathway receptors and cancer. The recipient of several grants, Dr. Mus-Veteau has served as a member of the CNRS evaluation Committee, responsible for the evaluation of labs and permanent researcher's activity and the recruitment of permanent researchers.
Bibliographische Angaben
- 2014, 2014, XXIII, 425 Seiten, 72 farbige Abbildungen, Maße: 16,3 x 24,3 cm, Gebunden, Englisch
- Herausgegeben: Isabelle Mus-Veteau
- Verlag: Springer, Berlin
- ISBN-10: 1493906615
- ISBN-13: 9781493906611
Sprache:
Englisch
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