Mouse Models of Allergic Disease

Transgenic Mouse Models.- Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture.- Evaluation of T Cell Function in Allergic Disease.- Evaluating B-Cells: From Bone Marrow Precursors to Antibody Producing Cells.- Protocols for Identifying, Enumerating and Assessing Mouse Eosinophils.- Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages.- Applications of Mouse Airway Epithelial Cell Culture for Asthma Research.- Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases.- Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE.- Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice.- Contact Hypersensitivity Models in Mice.- Induction of Allergic Rhinitis in Mice.- Induction of Allergic Airway Disease Using House Dust Mite Allergen.- An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airways Disease.- PAMPs and DAMPs in Allergy Exacerbation Models.- Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics.- Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma.- Clara Epithelial Cell Depletion in the Lung.- A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma.- Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings.- Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection.- Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes.- Flow Cytometric Methods for the Assessment of Allergic Disease.- Generation of Bone Marrow and Fetal Liver Chimeric Mice.

"This book is a collection of beautifully written chapters that are suitable to explore in-depth use of mouse models to understand allergic disease. ... This is a must read book for trainees, clinicians and researchers engaged in the study of allergy related manifestations. Undoubtedly, this book will provide an in-depth understanding regarding the use of mouse in allergic research. Using this invaluable text, various key phenomena behind allergic reactions can be explored." (Sandeep Kumar, World Allergy Organization, worldallergy.org, May, 2015)