Plant Fungal Pathogens

Fungal ABC Transporter Deletion and Localization Analysis.- Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation.- Activity-based Protein Profiling of Infected Plants.- The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves.- Proteomic Techniques for Plant-Fungal Interactions.- Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector.- Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens.- Real-time PCR for the Quantification of Fungi in planta.- Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi.- Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates.- Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors.- Characterization of Plant - Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens.- Heterologous Production of Fungal Effectors in Pichia pastoris.- The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta.- Metabolomics Protocols for Filamentous Fungi.- Targeted Gene Replacement in Fungi Using a Split-Marker Approach.- Massively Parallel Sequencing Technology in Pathogenic Microbes.- Confocal Microscopy in Plant Pathogen Interactions.- The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data.- Population Biology of Fungal Plant Pathogens.- Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens.- In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi.- Fungicide Resistance Assays for Fungal Plant Pathogens.- Identification ofLipid-binding Effectors.- In silico Identification and Characterization of Effector Catalogs.-

From the reviews:

"The current volume is a unique collection of 41 chapters from a diverse group of scientists, researchers, and academics working on different aspects of the molecular basis of fungal pathogens and pathogenesis. ... This volume will be useful for both students and researchers in the fields of botany and forestry, agriculture, mycology, microbiology, plant disease and diagnostics, molecular plant pathology, horticulture science, biomolecular sciences, life sciences, fungal molecular biology, fungal biotechnology, microbial biotechnology, and food technology." (S. K. Basu, Plant Science Bulletin, Vol. 59 (1), 2013)

From the reviews:

"The authors provide the latest information on topics including methods in molecular biology and highlighting some of the latest techniques critical for students pursuing molecular plant pathology. ... All the chapters are well illustrated with charts and graphs and with necessary photographs. ... should be available in school libraries experienced fungal research laboratories and university libraries. This is specially aimed at researchers new to the molecular biology field." (Asha Janadaree Dissanayake and Kevin D. Hyde, Fungal Diversity, Vol. 59, March, 2013)
"The current volume is a unique collection of 41 chapters from a diverse group of scientists, researchers, and academics working on different aspects of the molecular basis of fungal pathogens and pathogenesis. ... This volume will be useful for both students and researchers in the fields of botany and forestry, agriculture, mycology, microbiology, plant disease and diagnostics, molecular plant pathology, horticulture science, biomolecular sciences, life sciences, fungal molecular biology, fungal biotechnology, microbial biotechnology, and food technology." (S. K. Basu, Plant Science Bulletin, Vol. 59 (1), 2013)