Plant Metabolomics

Practical Applications of Metabolomics in Plant Biology.-Aspects of Experimental Design for Plant Metabolomics Experiments and Guidelines for Growth of Plant Material.-Separating the Inseparable: The Metabolomic Analysis of Plant - Pathogen Interactions.-Precautions for Harvest, Sampling, Storage and Transport of Crop Plant Metabolomics Samples.-Tissue Preparation using Arabidopsis.-Solid Phase Micro-Extraction (SPME) GC - MS Analysis of Natural Volatile Components in Melon and Rice.-Profiling Primary Metabolites of Tomato Fruit with Gas Chromatography/Mass-Spectrometry.-High Performance Liquid Chromatography (HPLC)- Mass Spectrometry (MS) Analysis of Plant Metabolites in Brassicaceae.-UPLC-MS-based Metabolite Analysis in Tomato.-High Precision Measurement and Fragmentation Analysis for Metabolite Identification.-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) for Plant Metabolite Profiling and Metabolite Identification.-Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics.-ICP-MS and LC-ICP-MS for Analysis of Trace Element Content and Speciation in the Cereal Grain.-The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses.-Data (pre-)processing of Nominal and Accurate Mass LC-MS or GC-MS Data using MetAlign.-TagFinder-Preprocessing Software for the Fingerprinting and the Profiling of Gas Chromatography-Mass Spectrometry(GC-MS) Based Metabolome Analyses.-Chemical Identification Strategies using Liquid Chromatography-photo Diode Array-solid PhaseExtraction-nuclear Magnetic Resonance / Mass Spectrometry.-A Strategy for Selecting Data Mining Techniques in Metabolomics.

From the reviews:

"The chapters presented in this volume stay true to the Methods and Protocols series, with a short piece of background information, a highly detailed step-by-step protocol, and additional practical information that will allow beginners in the field to set up repeatable, high-quality metabolomics experiments. ... this is an excellent addition to the series and can provide muchneeded support, especially to scientists starting in the growing field of plant metabolomics." (Alexandre Foito, Annals of Botany, Vol. 113 (7), April, 2014)"The book 'Plant Metabolomics. Methods and Protocols' is fresh in ideas and a useful manual for scientists, researchers, scholars, students and the wider readership interested in plant metabolism and metabolite molecules. ... The purpose of this volume is to provide information on basic practical questions concerning experimental work with metabolomics. ... The book is a mix between a scientific treatise and a laboratory manual. As such it is an excellent one." (Tadeusz Aniszewski, Plant Science Bulletin, Vol. 58 (4), 2012)