Plastids
Methods and Protocols
(Sprache: Englisch)
This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The...
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Klappentext zu „Plastids “
This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.
Inhaltsverzeichnis zu „Plastids “
Preface...Table of Contents...
Contributing Authors...
Part I Plastid Evolution, Plasticity, Structural, and Functional Diversity
1. Primary Endosymbioses: Emergence of the Primary Chloroplast and the Chromatophore, Two Independent Events
Eric Maréchal
2. Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events
Zoltán Füssy and Miroslav Oborník
3. Complex Endosymbiosis II: The Non-Photosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism
Cyrille Y. Botté and Yoshiki Yamaryo- Botté
4. Diversity and Plasticity of Plastids in Land Plants
Katalin Solymosi, Johanna Lethin, and Henrik Aronsson
5. The Main Functions of Plastids
Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Sali, and Marcel Kuntz
6. Plastid Transient and Stable Interactions with Other Cell Compartments
Stefanie J. Mueller-Schuessele and Morane Michaud
Part II Laboratory Protocols
7. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy
Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, and Denis Falconet
8. Purification of Chloroplasts and Chloroplast Subfractions: Envelope, Thylakoids, and Stroma - From Spinach, Pea, and Arabidopsis thaliana
Maryse A. Block and Catherine Albrieux
9. Isolation of Inner and Outer Membranes of the Chloroplast Envelope from Spinach and Pea
Maryse A. Block
10. Purification of Non-Green Plastids (Proplastids and Amyloplasts) from Angiosperms, and Isolation of Their Envelope Membranes
Claude Alban and Etienne-Pascal Journet
11. In Vitro Protein Import into Isolated Chloroplasts
Maryse A. Block
12. Analysis of the MTL Super-Complex at Contact Sites between Mitochondria and
... mehr
Plastids
Morgane Michaud
13. Isolation of Plastid-Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum Alexander Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Rio Bartulos
14. Isolating the Plasmodium falciparum Apicoplast using Magnetic Beads
Cyrille Y. Botté, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botté
15. Extraction and Quantification of Lipids from Plant or AlgaeValérie Gros, Josselin Lupette, and Juliette Jouhet
16. Quantitative Assessment of the Chloroplast Lipidome
Valérie Gros and Juliette Jouhet
17. Determination of the DNA/RNA-Associated Sub-Proteome from Chloroplasts and Other Plastid Types
Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt
18. Mapping Plastid Transcript Population by Circular Reverse Transcription-Polymerase Chain Reaction
Florence Courtois and Livia Merendino
19. Bioinformatic Analysis of Chloroplast Gene Expression and RNA Post-Transcriptional Maturations using RNA Sequencing
Bastien Malbert, Guillen Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy
20. A Guide to the Chloroplast Transcriptome Analysis using RNA-Seq
Elena J.S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoît Castandet
21. A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae
Águila Ruiz-Sola and Dimitris Petroutsos
22. Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants
Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day
23. Genetic Analysis of Chloroplast Biogenesis, and Function and Mutant Collections
Sho Fujii, Hajime Wada, and Koichi Kobayashi
24. Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum
Guillaume Allorent, Erika Guglielmino, Cécile Giustini, and Florence Courtois
Part III In Silico Tools25. In Silico Tools for the Prediction of Protein Import into Secondary Plastids
Daniel Moog
26. AT_CHLORO - The First Step When Looking for Information about Subplastidial Localization of Proteins
Daniel Salvi, Sylvain Bournais, Lucas Moyet, Imen Bouchnak, Marcel Kuntz, Christophe Bruley, and Norbert Rolland
Morgane Michaud
13. Isolation of Plastid-Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum Alexander Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Rio Bartulos
14. Isolating the Plasmodium falciparum Apicoplast using Magnetic Beads
Cyrille Y. Botté, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botté
15. Extraction and Quantification of Lipids from Plant or AlgaeValérie Gros, Josselin Lupette, and Juliette Jouhet
16. Quantitative Assessment of the Chloroplast Lipidome
Valérie Gros and Juliette Jouhet
17. Determination of the DNA/RNA-Associated Sub-Proteome from Chloroplasts and Other Plastid Types
Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt
18. Mapping Plastid Transcript Population by Circular Reverse Transcription-Polymerase Chain Reaction
Florence Courtois and Livia Merendino
19. Bioinformatic Analysis of Chloroplast Gene Expression and RNA Post-Transcriptional Maturations using RNA Sequencing
Bastien Malbert, Guillen Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy
20. A Guide to the Chloroplast Transcriptome Analysis using RNA-Seq
Elena J.S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoît Castandet
21. A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae
Águila Ruiz-Sola and Dimitris Petroutsos
22. Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants
Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day
23. Genetic Analysis of Chloroplast Biogenesis, and Function and Mutant Collections
Sho Fujii, Hajime Wada, and Koichi Kobayashi
24. Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum
Guillaume Allorent, Erika Guglielmino, Cécile Giustini, and Florence Courtois
Part III In Silico Tools25. In Silico Tools for the Prediction of Protein Import into Secondary Plastids
Daniel Moog
26. AT_CHLORO - The First Step When Looking for Information about Subplastidial Localization of Proteins
Daniel Salvi, Sylvain Bournais, Lucas Moyet, Imen Bouchnak, Marcel Kuntz, Christophe Bruley, and Norbert Rolland
... weniger
Bibliographische Angaben
- 2018, 1st ed. 2018, XV, 409 Seiten, 46 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Eric Maréchal
- Verlag: Springer, Berlin
- ISBN-10: 1493986538
- ISBN-13: 9781493986538
- Erscheinungsdatum: 10.07.2018
Sprache:
Englisch
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