Practical Pharmacology for the Pharmaceutical Sciences

Preface ix Acknowledgements xi 1 Before Entering the Pharmacology Laboratory 1 1.1 Safety and Risk Assessment 1 1.2 The Laboratory Record Book 3 1.3 Use of Animals in Practical Pharmacology 4 1.4 Experimental Design 5 1.5 Units, Dilutions and Logarithms 7 1.5.1 Units of Mass 8 1.5.2 Units, Concentrations and Logarithms 8 1.5.3 Dilutions 9 1.5.4 Logarithms 10 1.6 Essential Statistics 12 1.6.1 Continuous Data - t-test, ANOVA, Non-parametric Tests and Regression 12 1.6.2 Discontinuous Data - X2 and Fisher's Exact Test 21 2 Basic Pharmacological Principles 27 2.1 Drug-Receptor Interaction 27 2.1.1 Agonists 27 2.1.2 Antagonists 30 2.1.3 Receptor Classification 36 2.2 Bioassays 37 2.2.1 Single-point Assays 38 2.2.2 Bracketing Assays, Three-point or 2×1 Assays 38 2.2.3 Multi-point Assays, Such As Four-point or 2×2 Assays 39 3 Isolated Tissues and Organs 43 3.1 Equipment for In Vitro Experiments 44 3.2 Organ Baths 45 3.3 Physiological Salt Solutions 46 3.4 Transducers 47 3.5 Recording Equipment and Software 49 3.6 Dosing 50 3.7 Electrically Stimulated Preparations 52 3.8 Fault-Finding of In Vitro Isolated Tissue Preparations 53 4 Smooth Muscle Preparations 55 4.1 Gastrointestinal Smooth Muscle Preparations 55 4.2 Guinea Pig Isolated Ileum 56 4.2.1 Concentration-Response Curves for Cholinesters 57 4.2.2 Selective Antagonism 59 4.2.3 Specificity of Blood Cholinesterases 62 4.2.4 Quantification of the Potency of an Antagonist 64 4.2.5 Bioassays 67 4.2.6 Calcium Channel Blockers 73 4.2.7 Field-stimulated Guinea Pig Isolated Ileum 76 4.3 Rabbit Isolated Jejunum and the Finkleman Preparation 78 4.3.1 Adrenoceptor Sub-types 79 4.4 Isolated Tracheal Rings 80 4.5 Isolated Vas Deferens 82 5 Cardiovascular Preparations 93 5.1 Isolated Perfused Heart Preparations 94 5.1.1 The Langendorff Preparation 95 5.1.2 Cardiac Interactions of Anti-asthma Drugs 98 5.1.3 The Rat Isolated Auricle Preparation 99 5.2 Thoracic Aorta Preparation 102 5.2.1 Drugs Regulating Nitric Oxide-mediated 6

"This is a unique, well-written book that covers everything an educator would need to set up and run a basic laboratory course in pharmacology." ( Doody s , 23 January 2015)

"Supported with numerous questions throughout the text, as well as step by step instructions for practical experiments, this book presents an approach to learning pharmacology through an appreciation of authentic experimental data." ( Kingbook73 s Medical Ebook and Video Collection , 8 October 2014)