Protein and Peptide Mass Spectrometry in Drug Discovery
(Sprache: Englisch)
With chapters provided by international leading experts, this book covers the recent advances in protein and peptide mass spectrometry. Focusing on the pharmaceutical industry, it addresses both emerging techniques, including imaging mass spectrometry, ion...
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Produktinformationen zu „Protein and Peptide Mass Spectrometry in Drug Discovery “
With chapters provided by international leading experts, this book covers the recent advances in protein and peptide mass spectrometry. Focusing on the pharmaceutical industry, it addresses both emerging techniques, including imaging mass spectrometry, ion mobility, and microwave-assisted mass spectrometry, and recent applications, including pharmaceutical analysis throughout the drug development cycle. The book stresses practice and applications, providing real world examples from industry contributors. After overviewing methodology and discussing recent studies, the remaining chapters address newer techniques for determining protein structure, interactions with peptides, proteins, and ligands, and protein folding and unfolding.
Klappentext zu „Protein and Peptide Mass Spectrometry in Drug Discovery “
The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discoveryAn instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery.
Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.
The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery An instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery.
Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.d techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery.
Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical indu
Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.d techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery.
Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical indu
Inhaltsverzeichnis zu „Protein and Peptide Mass Spectrometry in Drug Discovery “
- Preface- Contributors
Part I. Methodology
1.Ionization Methods in Protein Mass Spectrometry (Ismael Cotte-Rodriguez, Yun Zhang, Zhixin Miao and Hao Chen)
1.1 History of the development of protein mass spectrometry
1.2 Laser based ionization methods for proteins
1.3 Spray-based ionization methods for proteins
1.4 Ambient ionization methods
1.5 Conclusions
2. Ion Activation and Mass Analysis in Protein Mass Spectrometry (Cheng Lin and Peter O'Connor)
2.1 Introduction
2.2 Ion Activation and Tandem MS Analysis
2.3 Mass Analyzers
3. Target Proteins: Bottom-up and Top-down Proteomics (Michael Boyne and Ron Bose)
3.1 Mass Spectral Approaches to Targeted Protein Identification
3.2 Bottom-up Proteomics
3.3 Top-down Approaches
3.4 Next generation approaches
4. Quantitative Proteomics by Mass Spectrometry (Jacob Galan, Anton Iliuk and W. Andy Tao)
4.1 Introduction
4.2 In-cell Labeling
4.3 Quantitation via Isotopic Labeling of Proteins
4.4 Quantitation via Isotopic Labeling on Peptides
4.5 Label-free Quantitation
4.6 Conclusions
4.7 Acknowledgment
5. Comparative Proteomics by Direct Tissue Analysis using Imaging Mass Spectrometry (Michelle L. Reyzer and Richard M. Caprioli)
5.1 Introduction
5.2 Conventional Comparative Proteomics
5.3 Comparative Proteomics using Imaging MS
5.4 Conclusions
5.5 Acknowledgements
6. Peptide and Protein Analysis using Ion Mobility-Mass Spectrometry (Jeffrey R. Enders, Michal Kliman, Sevugarajan Sundarapandian and John A. McLean)
6.1 Ion Mobility-Mass Spectrometry: Instrumentation and Separation Selectivity
6.2 Characterizing and Interpreting Peptide and Protein Structure
6.3 Applications of IM-MS to Peptide and Protein Characterization
6.4 Future Directions
7. Chemical Footprinting for Determining Protein Properties and Interactions (Sandra A. Kerfoot and Michael L. Gross)
7.1 Introduction to Hydrogen/Deuterium Exchange
7.2 Experimental Procedures
7.3 Mass Spectrometry-based HDX in Practice
7.4 Protein
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footprinting via Free-radical Oxidation
7.5 Chemical Cross Linking
7.6 Selective and Irreversible Chemical Modification
7.7 Conclusion
8. Microwave Technology to Accelerate Protein Analysis (Urooj A. Mirza, Birendra N. Pramanik and Ajay K. Bose)
8.1 Introduction
8.2 Microwave Technology
8.3 Summary
8.4 Acknowledgements
9. Bioinformatics and Database Searching (Surendra Dasari and David L. Tabb)
9.1 Overview
9.2 Introduction to Tandem Mass Spectrometry
9.3 Overview of Peptide Identification with Database Searching
9.4 MyriMatch-IDPicker Protein Identification Pipline
9.5 Results of a Shotgun Proteomics Study
9.6 Improvements to MyriMatch DatabaseSearch Engine
9.7 Applications of MyriMatch-IDPicker Pipeline
9.8 Conclusion
9.9 Acknowledgements
Part II. Application
10. Mass Spectrometry-based Screening and Characterization of Protein - Ligand Complexes in Drug Discovery (Christine L. Andrews, Michael R. Ziebell, Elliott Nickbarg and Xianshu Yang)
10.1 Introduction
10.2 Affinity Selection Mass Spectrometry (AS-MS)
10.3 Solution-based AS-MS as Screening Technologies
10.4 Gas-Phase Interactions
10.5 Enzyme Activity Assays using MS for Screening or Confirming Drug Candidates
10.6 Conclusions and Future Directions
11. Utilization of Mass Spectrometry for the Structural Characterization of Biopharmaceutical Protein Products (Amareth Lim and Catherine A. Srebalus Barnes)
11.1 Introduction
11.2 MS-Based Approach for the Characterization of Recombinant Therapeutic Proteins
11.3 Cell Culture Development
11.4 Purification Development
11.5 Formulation Development
11.6 Analytical Method Development
11.7 Confirmation of Structure/Product Comparability Assessment
11.8 Conclusions
12. Post-Translationally Modified Proteins: Glycosylation, Phosphorylation, and Disulfide Bond Formation (Anthony Tsarbopoulos and Fotini N. Bazoti)
12.1 Introduction
12.2 Glycosylation
12.3 Phosphorylation
12.4 Disulfide Bond Detection and Mapping
12.5 Future Perspectives
13. Mass Spectrometry of Antigenic Peptides (Henry Rohrs)
13.1 Introduction
13.2 Analysis of Antigenic Peptides
13.3 Examples of the Application of Mass Spectrometry to Antigenic Peptide Study
13.4 Future
14. Neuropeptidomics Jonathan V. Sweedler, Fang Xie and Adriana Bora)
14.1 Introduction
14.2 Neuropeptidomics: Characterizing Peptides in the Brain
14.3 Sample Preparation for Mass Spectrometry
14.4 Separations
14.5 Peptide Characterization via Mass Spectrometry
14.6 Conclusions
14.7 Future Perspectives
14.8 Acknowledgements
15. Mass Spectrometry for the Study of Peptide Drug Metabolism (Patrick J. Rudewics)
15.1 Introduction
15.2 Peptide Drug Metabolism
15.3 LC-MS/MS for Metabolite Identification
15.4 Quantitative Analysis
15.5 Case Study: Il-1ß Protease Inhibitors
15.6 Future Directions Detection and Mapping
12.5 Future Perspectives
13. Mass Spectrometry of Antigenic Peptides (Henry Rohrs)
13.1 Introduction
13.2 Analysis of Antigenic Peptides
13.3 Examples of the Application of Mass Spectrometry to Antigenic Peptide Study
13.4 Future
14. Neuropeptidomics Jonathan V. Sweedler, Fang Xie and Adriana Bora)
14.1 Introduction
14.2 Neuropeptidomics: Characterizing Peptides in the Brain
14.3 Sample Preparation for Mass Spectrometry
14.4 Separations
14.5 Peptide Characterization via Mass S
7.5 Chemical Cross Linking
7.6 Selective and Irreversible Chemical Modification
7.7 Conclusion
8. Microwave Technology to Accelerate Protein Analysis (Urooj A. Mirza, Birendra N. Pramanik and Ajay K. Bose)
8.1 Introduction
8.2 Microwave Technology
8.3 Summary
8.4 Acknowledgements
9. Bioinformatics and Database Searching (Surendra Dasari and David L. Tabb)
9.1 Overview
9.2 Introduction to Tandem Mass Spectrometry
9.3 Overview of Peptide Identification with Database Searching
9.4 MyriMatch-IDPicker Protein Identification Pipline
9.5 Results of a Shotgun Proteomics Study
9.6 Improvements to MyriMatch DatabaseSearch Engine
9.7 Applications of MyriMatch-IDPicker Pipeline
9.8 Conclusion
9.9 Acknowledgements
Part II. Application
10. Mass Spectrometry-based Screening and Characterization of Protein - Ligand Complexes in Drug Discovery (Christine L. Andrews, Michael R. Ziebell, Elliott Nickbarg and Xianshu Yang)
10.1 Introduction
10.2 Affinity Selection Mass Spectrometry (AS-MS)
10.3 Solution-based AS-MS as Screening Technologies
10.4 Gas-Phase Interactions
10.5 Enzyme Activity Assays using MS for Screening or Confirming Drug Candidates
10.6 Conclusions and Future Directions
11. Utilization of Mass Spectrometry for the Structural Characterization of Biopharmaceutical Protein Products (Amareth Lim and Catherine A. Srebalus Barnes)
11.1 Introduction
11.2 MS-Based Approach for the Characterization of Recombinant Therapeutic Proteins
11.3 Cell Culture Development
11.4 Purification Development
11.5 Formulation Development
11.6 Analytical Method Development
11.7 Confirmation of Structure/Product Comparability Assessment
11.8 Conclusions
12. Post-Translationally Modified Proteins: Glycosylation, Phosphorylation, and Disulfide Bond Formation (Anthony Tsarbopoulos and Fotini N. Bazoti)
12.1 Introduction
12.2 Glycosylation
12.3 Phosphorylation
12.4 Disulfide Bond Detection and Mapping
12.5 Future Perspectives
13. Mass Spectrometry of Antigenic Peptides (Henry Rohrs)
13.1 Introduction
13.2 Analysis of Antigenic Peptides
13.3 Examples of the Application of Mass Spectrometry to Antigenic Peptide Study
13.4 Future
14. Neuropeptidomics Jonathan V. Sweedler, Fang Xie and Adriana Bora)
14.1 Introduction
14.2 Neuropeptidomics: Characterizing Peptides in the Brain
14.3 Sample Preparation for Mass Spectrometry
14.4 Separations
14.5 Peptide Characterization via Mass Spectrometry
14.6 Conclusions
14.7 Future Perspectives
14.8 Acknowledgements
15. Mass Spectrometry for the Study of Peptide Drug Metabolism (Patrick J. Rudewics)
15.1 Introduction
15.2 Peptide Drug Metabolism
15.3 LC-MS/MS for Metabolite Identification
15.4 Quantitative Analysis
15.5 Case Study: Il-1ß Protease Inhibitors
15.6 Future Directions Detection and Mapping
12.5 Future Perspectives
13. Mass Spectrometry of Antigenic Peptides (Henry Rohrs)
13.1 Introduction
13.2 Analysis of Antigenic Peptides
13.3 Examples of the Application of Mass Spectrometry to Antigenic Peptide Study
13.4 Future
14. Neuropeptidomics Jonathan V. Sweedler, Fang Xie and Adriana Bora)
14.1 Introduction
14.2 Neuropeptidomics: Characterizing Peptides in the Brain
14.3 Sample Preparation for Mass Spectrometry
14.4 Separations
14.5 Peptide Characterization via Mass S
... weniger
Autoren-Porträt von Gross, Chen
Michael L. Gross, PhD, is a Professor in the Departments of Chemistry, Medicine, and Immunology at Washington University in St. Louis. He is also Principal Investigator at the National Institutes of Health (NIH) Research Resource in Mass Spectrometry.Guodong Chen, PhD, is Principal Scientist in Bioanalytical and Discovery Analytical Sciences at Bristol-Myers Squibb in Princeton, New Jersey. He heads a mass spectrometry group in support of drug discovery as well as development programs in small molecule pharmaceuticals and biologics.
Birendra N. Pramanik, PhD, was a Distinguished Fellow at Schering-Plough Research Institute (SPRI), where he directed the spectroscopy, mass spectrometry, and NMR programs.
Rezension zu „Protein and Peptide Mass Spectrometry in Drug Discovery “
"This book will be a valuable reference as it contains plenty of depth and substance to be of interest to experienced practitioners of mass spectrometry and related techniques, but is still accessible to pharmaceutical researchers who want to learn more about MS technologies and its applications." (American Society for Mass Spectrometry, 1 July 2012)
Pressezitat
"This book will be a valuable reference as it contains plenty of depth and substance to be of interest to experienced practitioners of mass spectrometry and related techniques, but is still accessible to pharmaceutical researchers who want to learn more about MS technologies and its applications." (American Society for Mass Spectrometry, 1 July 2012)
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