Protocols for the Diagnosis of Pig Viral Diseases

Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms.- Collection of Samples, Their Preservation, and Transportation.- Methods for Quantification of Viruses.- Protocols for Isolation of Genetic Materials from RNA Viruses.- Multiplex PCR for Diagnosis of Porcine Diseases.- Protocols for Isolation of Plasmid DNA.- Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases.- RNA-PAGE-Based Diagnosis of Viral Diseases.- Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases.- Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases.- Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance.- Antibody-Based Sensors for Pathogen Detection.- Lateral Flow Assay for Diagnosis of Pig Viral Diseases.- Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases.- Protocols for Immunofluorescence Techniques.- Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases.- Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases.- Cell Culture System for Porcine Virus Isolation and Propagation.- An Overview of Mouse Monoclonal Antibody Production.- Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective.- Ligase Detection Reaction-Fluorescent Microsphere Assay.- ELISA as a Diagnostic Weapon.- SDS-PAGE and Western Blotting: Basic Principles and Protocol.- Immune Assays as Diagnostic for Pig Viral Diseases.- Production of Virus-Like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis.- Good Laboratory Practices and Biosafety Containments in a Virology Laboratory.

Dr. Rajib Deb, PhD is currently employed as Scientist at Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, India. He has served more than 9 years at Agricultural Research Service, India. He completed his post-graduation and PhD at Indian Veterinary Research Institute, Uttar Pradesh, India in the subject of Animal Biotechnology. He is currently pursuing the National Post-Doctoral Fellowship program at National Dairy Research Institute, Karnal, Haryana, India. He has published more than 35 research papers in peer reviewed journals. He is the recipient of several National and International Awards including TWAS Fellowship, Italy; Associate of National Academy of Agricultural Sciences, India; Member of National Academy of Sciences, India; Member of Indian National Young Academy of Sciences, India. He has filed 9 Indian Patents and developed several technologies. He is currently working on the development of diagnostics and vaccines against porcine disease.
Dr. Ajay Kumar Yadav, PhD is the scientist in veterinary virology at Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, India. He has completed graduation from Govind Ballabh Pant University of Agriculture and Technology, Uttarakhand, India. Later on, he completed his M.V. Sc. and PhD in veterinary virology from the premier veterinary institute named Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Uttar Pradesh, India. Dr. Yadav joined the Agricultural Research Services (ARS) in the discipline of veterinary microbiology in the year 2018. His research interests include disease diagnosis, cell culture, and virus isolation. He is a member of several societies, and has also published more than 20 research articles in peer-reviewed international and national journals. He has more than 60 GenBank submissions.
Dr. Swaraj Rajkhowa, PhD joined Agricultural Research Service (ARS)