Puumala and Dobrava Hantaviruses
Development of PCR Protocols
(Sprache: Englisch)
Throughout the world, nearly 150.000 persons are infected with hantaviruses each year. Hantaviruses are divided into two groups based on their human pathogenicity: Old World hantaviruses causing hemorrhagic fever with renal syndrome (HFRS) and New World...
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Throughout the world, nearly 150.000 persons are infected with hantaviruses each year. Hantaviruses are divided into two groups based on their human pathogenicity: Old World hantaviruses causing hemorrhagic fever with renal syndrome (HFRS) and New World hantaviruses causing hantavirus pulmonary syndrome (HPS). HFRS is an acute disease characterised by vascular bleeding and kidney damage, and, in most cases, it is not lethal. Mild forms of HFRS are usually caused by Puumala virus (PUUV), while the most severe forms of HFRS in Europe are caused by Dobrava-Belgrade virus (DOBV). Therefore, sensitive and specific molecular detection is essential for the timely diagnosis of hantavirus infections. In a typical laboratory context, we present the creation of nested, degenerate primers for detection of S and M segment of DOBV and M segment of PUUV, optimisation of various PCR components and cycling parameters, using both external and internal primers. As a result, this book provides a resource for anyone interested in conducting similar experiments and procedures.
Autoren-Porträt von Lejla Smajlovic Skenderagic, Mirsada Hukic
Smajlovic Skenderagic, LejlaAssist. Prof. Lejla Smajlovic Skenderagic is a lecturer at International Burch University in Sarajevo, with research interests in forensic and virus genetics. Prof. Mirsada Hukic, MD PhD, is the director of the CDC and GeoHealth Studies at ANUBIH and International Society for Hantaviruses Council member, with over 40 years of research experience.
Bibliographische Angaben
- Autoren: Lejla Smajlovic Skenderagic , Mirsada Hukic
- 2021, 84 Seiten, Maße: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 3659926531
- ISBN-13: 9783659926532
Sprache:
Englisch
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