Quantitative Detection of E.coli O157:H7 in Ground Beef
(Sprache: Englisch)
Escherichia coli O157:H7 is recognized as a major human enteropathogen. A direct spectrophotometric immune agglutination - the first assay had ever published - was developed for quantitation of specific Escherichia coli O157 IgG. A new cell lysis solution...
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Escherichia coli O157:H7 is recognized as a major human enteropathogen. A direct spectrophotometric immune agglutination - the first assay had ever published - was developed for quantitation of specific Escherichia coli O157 IgG. A new cell lysis solution designated TZ was also developed and found superior to a variety of cell lysing methods prior to PCR. A 6-fold increase of extracted E. coli 0157:H7 cells from seeded in ground beef was achieved via developing a differential extraction procedure. Highest amplification of E. coli O157: H7 was achieved with the aid of TZ lysis solution, extraction procedures and the optimization of PCR procedure; resulted in the detection of 100 cells of SLT-1 and 50 cells with SLT-2 per 10 grams of ground beef.
Autoren-Porträt von Assem Abolmaaty Sayedahmed
Abolmaaty Sayedahmed, AssemAssociate Professor, Food Microbiology and Safety, Former Vice-Director, Microbial Resource Center (Cairo MIRCEN), Former director, Technology & Innovation Offices, Former director, international collaboration office, Ain Shams University, Cairo, Egypt, Ph.D degree from University of Massachusetts.
Bibliographische Angaben
- Autor: Assem Abolmaaty Sayedahmed
- 2019, 216 Seiten, Maße: 22 cm, Kartoniert (TB), Englisch
- Verlag: LAP Lambert Academic Publishing
- ISBN-10: 6139971837
- ISBN-13: 9786139971831
Sprache:
Englisch
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