Retinal Degeneration
Methods and Protocols
(Sprache: Englisch)
This Methods in Molecular Biology(TM) book offers methods for studying retinal homeostasis and disease, including cell culture systems and animal models of disease, their generation, phenotypic and molecular characterization and use in therapeutic approaches.
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Produktdetails
Produktinformationen zu „Retinal Degeneration “
This Methods in Molecular Biology(TM) book offers methods for studying retinal homeostasis and disease, including cell culture systems and animal models of disease, their generation, phenotypic and molecular characterization and use in therapeutic approaches.
Klappentext zu „Retinal Degeneration “
Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.
Inhaltsverzeichnis zu „Retinal Degeneration “
Identification and Analysis of Inherited Retinal Disease Genes.-Mouse Models for Studies of Retinal Degeneration and Diseases.-Retinal Fundus Imaging in Mouse Models of Retinal Diseases.-Functional Phenotyping of Mouse Models with ERG.-Phenotyping of Mouse Models with OCT.-Light Damage as a Model of Retinal Degeneration.-N-Methyl-D-Aspartate (NMDA)-mediated Excitotoxic Damage - A Mouse Model of Acute Retinal Ganglion Cell Damage.-Generation of Transgenic X. laevis Models of Retinal Degeneration.-Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio.-Analysis of Optokinetic Response in Zebrafish by Computer Based Eye Tracking.-Analysis of the Drosophila Compound Eye with Light and Electron Microscopy.-Cell Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy.-A Method of Horizontally-Sliced Preparation of the Retina.-Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL.-High-throughput RNA in situ Hybridization in Mouse Retina.- Assessment of Mitochondrial Damage in Retinal Cells and Tissues using Quantitative Polymerase Chain Reaction for Mitochondrial DNA Damage and Extracellular Flux Assay for Mitochondrial Respiration Activity.-Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells in situ.-Ca2+ Microfluorimetry in Retinal Müller Glial Cells.-Functional Analysis of Retinal Microglia and their Effects on Progenitors.-Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture.-Ca2+ Imaging Techniques to Analyze Ca2+ Signaling in Cells and to Monitor Neuronal Activity in the Retina.-Double Chromatin Immunoprecipitation:Analysis of Target Co-Occupancy of Retinal Transcription Factors.-Quantifying the Activity of cis-regulatory Elements in the Mouse Retina by Explant Electroporation.-Optimized Technique for Subretinal Injections in Mice.-Adeno Associated Viral Vectors for Gene Therapy of Inherited Retinal Degenerations.-Barrier Modulation in Drug Delivery to the Retina.
Bibliographische Angaben
- 2016, Softcover reprint of the original 1st ed. 2013, XIV, 384 Seiten, 48 farbige Abbildungen, Maße: 17,8 x 25,4 cm, Kartoniert (TB), Englisch
- Herausgegeben: Bernhard H.F. Weber, Thomas Langmann
- Verlag: Springer, Berlin
- ISBN-10: 1493959697
- ISBN-13: 9781493959693
Sprache:
Englisch
Pressezitat
From the book reviews:"This step-by-step guide to studying retinal cell biology in health and disease covers a large spectrum of techniques and animal model species. ... Certainly, this book is for basic scientists. It is a great tool for those based in laboratories, and researchers at all levels can benefit from the basics to key tips on troubleshooting cutting-edge techniques and avoiding pitfalls. ... This book is great in simplifying new cutting-edge techniques in studying retinal degeneration." (Ayham Skaf, Doody's Book Reviews, May, 2013)
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