Retinal Development

Conditional Control of Gene Expression in the Mouse Retina.-Generation of Transgenic Xenopus Using Restriction Enzyme-Mediated Integration.-In vivo Functional Analysis of Transcription Factor--response Element Interaction using Transgenic Xenopus laevis.-Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes.-In Ovo Eye Electroporation.-Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos.-Testing Retina Fate Commitment in Xenopus by Blastomere Deletion, Transplantation and Explant Culture.-Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cells.-Fate Tracing of neurogenin2-expressing Cells in the Mouse Retina using CreERTM - LacZ.-In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development.-In vitro Biochemical Assays to Monitor Rhodopsin Function.-Transfection of Primary Embryonic Chicken Retinal Cells using Cationic Lipid.-Production of High Titer RCAS Retrovirus.-Chick RPE Transdifferentiation Assay for Proneural Activities.-Studying the Generation of Regenerated Retinal Neuron from Müller glia in the Mouse Eye.-Production and Transplantation of Retinal Cells from Human and Mouse Embryonic Stem Cells.-Light-induced Photoreceptor Degeneration in the Retina of the Zebrafish.-Microarray-based Gene Profiling Analysis of Müller Glia-derived Retinal Stem Cells in Light-damaged Retinas from Adult Zebrafish.-Measuring Rodent Electroretinograms to Assess Retinal Function.-Functional Imagingof Retinal Photoreceptors and Inner Neurons using Stimulus-evoked Intrinsic Optical Signals.-Use of Laser Capture Microdissection for Analysis of Retinal mRNA/miRNA Expression and DNA Methylation.-Revealing Looping Organization of Mammalian Photoreceptor Genes Using Chromosome Conformation Capture (3C) Assays.-Retinal Transcriptome Profiling by Directional Next Generation Sequencing Using 100 ng of Total RNA.-Exome Sequencing: Capture and