Retinoids

Quantification of Endogenous Retinoids.- Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids.- Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol.- In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists.- Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments.- Measurement of the Mobility of All-Trans-Retinol with Two-Photon Fluorescence Recovery After Photobleaching.- Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors.- HPLC?/?MSN Analysis of Retinoids.- Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration.- Fluorescence-Based Technique for Analyzing Retinoic Acid.- The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy.- Assay of Retinol-Binding Protein-Transthyretin Interaction and Techniques to Identify Competing Ligands.- Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle.- Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy.- Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues.- Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 Enzymes.- Diet in Vitamin A Research.- Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium.- Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina.- Techniques to StudySpecific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake.

From the reviews:"This excellent book provides authoritative and cutting-edge information in the field of retinoids ... . It is intended both for beginning and experienced researchers in diverse fields who are interested in learning new techniques. Even graduate students, nutritionists, and clinicians could find this book informative and helpful. ... By combining biochemical, biophysical, and cell biological techniques, the book thoroughly addresses the field of retinoid research." (Omer Iqbal, Doody's Review Service, October, 2010)