RNA-Protein Complexes and Interactions

Characterization ofRNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 andSRSF2.- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNAAffinity Purification).- Biotin-Streptavidin Affinity Purification ofRNA-Protein Complexes Assembled In Vitro.- Detecting RNA-Protein InteractionUsing End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting.- Purificationof RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro SplicingReaction Mixture.- Loading of Argonaute Protein with Small Duplex RNA inCellular Extracts.- In Vitro Analysis of Ribonucleoprotein Complex Remodelingand Disassembly.- Single-Turnover Kinetics of Methyl Transfer to tRNA byMethyltransferases.- Purification and Functional Reconstitution of Box H/ACARibonucleoprotein Particles.- Northwestern Blot Analysis: Detecting RNA-ProteinInteraction After Gel Separation of Protein Mixture.- Measuring mRNATranslation by Polysome Profiling.- Genome-Wide Profiling of RNA-ProteinInteractions Using CLIP-Seq.- Identification of Endogenous mRNA-BindingProteins in Yeast Using Crosslinking and PolyA Enrichment.- Ribo-ProteomicsApproach to Profile RNA-Protein and Protein-Protein Interaction Networks.- Detectionof Protein-Protein Interaction Within an RNA-Protein Complex ViaUnnatural-Amino-Acid-Mediated Photochemical Crosslinking.- Evolution ofCell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.- mCarts: Genome-WidePrediction of Clustered Sequence Motifs as Binding Sites for RNA BindingProteins.- Design of RNA-Binding Proteins: Manipulate Alternative Splicing inHuman Cells with Artificial Splicing Factors.- Informational Suppression toProbe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5' Splice-SiteBase-Pairing.- Analysis of Alternative Pre-RNA Splicing in the Mouse RetinaUsing a Fluorescent Reporter.