Structural Proteomics
High-Throughput Methods
(Sprache: Englisch)
The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in...
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Produktinformationen zu „Structural Proteomics “
The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. It is the first book of protocols to cover techniques in a new and emerging field.
Klappentext zu „Structural Proteomics “
The objective of this volume is to provide readers with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimised. This volume includes chapters describing, in-depth, the individual steps in the Structural Genomics pipeline, as well as less detailed overviews of individual Structural Genomics initiatives. It is the first book of protocols to cover techniques in a new and emerging field.
Inhaltsverzeichnis zu „Structural Proteomics “
- Target selection for structural genomics: An Overview.- A General target selection method for crystallographic proteomics.
- Target Selection: Triage in the Structural Genomics Battlefield.
- Data Management in Structural Genomics: An Overview.
- Data deposition and annotation at the Worldwide Protein Data Bank.
- Prediction of protein disorder.
- Protein domain prediction.
- Protein Structure Modeling With MODELLER.
- High throughput cloning with restriction enzymes.
- Automated recombinant protein expression screening in Escherichia coli.
- From no expression to high-level soluble expression in Escherichia coli by screening a library of the target protein with randomized N-terminus.
- Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli.
- A high-throughput platform for eukaryotic genes.
- High-Throughput production of recombinant human proteins for crystallography.
- Assembly of protein complexes by co-expression in prokaryotic and eukaryotic hosts.
- Cell-free protein synthesis for analysis by NMR spectroscopy.
- A medium or high-throughput protein refolding assay.
- Structural Proteomics of Membrane Proteins: A Survey of Published Techniques and Design of a Rational High-Throughput Strategy.
- Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multi-angle or static light scattering.
- High-Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram Scale.
- High Throughput Screening of Purified Proteins for Enzymatic Activity.
- Strategies for Improving Crystallization Success.
- Protein crystallization in restricted geometry: Advancing old ideas for modern times in structural proteomics.
- Fluorescence Approaches to Growing Macromolecule Crystals.
- Efficient Macromolecular Crystallization using Microfluidics and Randomized Design of Screening Reagents.
- Increasing protein crystallization success with heterogeneous
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nucleating agents.
- High-Throughput pH Optimization of Protein Crystallization.
- Automated Structure Solution with the PHENIX Suite.
- NMR Screening for Rapid Protein Characterization in Structural Proteomics.
- Micro-coil NMR spectroscopy - a novel tool for biological high-throughput NMR spectroscopy.
- Protein Structure Determination Using a Combination Of Cross-linking, Mass Spectrometry and Molecular Modeling.
- Structural Genomics of Minimal Organisms: Pipeline and Results.
- Structural Genomics of Pathogenic Protozoa: An Overview.
- High-Throughput Crystallography at SGC Toronto: an overview.
- The Structural Biology and Genomics Platform in Strasbourg: An overview.
- Bacterial Structural Genomics Initiative: Overview of Methods and Technologies Applied to the Process of Structure Determination.
- High-Throughput Protein Production and Crystallization at NYSGXRC.
- Overview of the Pipeline for Structural and Functional Characterization of Macrophage Proteins at the University of Queensland.
- Structural Genomics of the Bacterial Mobile Metagenome: An Overview.
- High-Throughput pH Optimization of Protein Crystallization.
- Automated Structure Solution with the PHENIX Suite.
- NMR Screening for Rapid Protein Characterization in Structural Proteomics.
- Micro-coil NMR spectroscopy - a novel tool for biological high-throughput NMR spectroscopy.
- Protein Structure Determination Using a Combination Of Cross-linking, Mass Spectrometry and Molecular Modeling.
- Structural Genomics of Minimal Organisms: Pipeline and Results.
- Structural Genomics of Pathogenic Protozoa: An Overview.
- High-Throughput Crystallography at SGC Toronto: an overview.
- The Structural Biology and Genomics Platform in Strasbourg: An overview.
- Bacterial Structural Genomics Initiative: Overview of Methods and Technologies Applied to the Process of Structure Determination.
- High-Throughput Protein Production and Crystallization at NYSGXRC.
- Overview of the Pipeline for Structural and Functional Characterization of Macrophage Proteins at the University of Queensland.
- Structural Genomics of the Bacterial Mobile Metagenome: An Overview.
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Bibliographische Angaben
- 2008, 628 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Bostjan Kobe, Thomas Huber, Mitchell Guss
- Verlag: Humana Press
- ISBN-10: 1588298094
- ISBN-13: 9781588298096
- Erscheinungsdatum: 03.03.2008
Sprache:
Englisch
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