The LINC Complex

EM Tomography of LINC Complexes.- Recombinant Purification of the Periplasmic Portion of the LINC Complex.- Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis.- Analysis of Nesprin-2 Interaction with its Binding Partners and Actin.- Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID.- Using Nesprin Tension Sensors to Measure Force on the LINC Complex.- Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei.- Direct Force Probe for Nuclear Mechanics.- Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning.- Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments.- Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy.- Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging.- Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans.- High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development.- Computational Methods for Studying the Plant Nucleus.- Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System.- Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components.- Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice.- An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle.- Functional Analysis of LINC Complexes in Skin.- Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer.- Next-generation