The Mitoribosome

Discovery of Mitochondrial Ribosomes.- Evolution: Mitochondrial Ribosomes across Species.- Mitoribosome Biogenesis.- Translation in Mitochondrial Ribosomes.- Sample Preparation of Isolated Mitochondria for Cryoelectron Tomography and in situ Studies of Translation.- Cryo-EM for Structure Determination of Mitochondrial Ribosome Samples.- Sucrose Gradient Analysis of Human Mitochondrial Ribosomes and RNA.- Yeast Mitoribosome Purification and Analyses by Sucrose Density Centrifugation and Immunoprecipitation.- Rapid Cryo-Purification of the Yeast Mitochondrial Ribosome.- Methods to Study the Biogenesis of Mitoribosomal Proteins in Yeast.- Systematic Analysis of Assembly Intermediates in Yeast to Decipher the Mitoribosome Assembly Pathway.- Metabolic Labelling of Mitochondrial Translation Products in Whole Cells and Isolated Organelles.- Assembly of the Mitochondrial Translation Initiation Complex.- Reconstitution of Mammalian Mitochondrial Translation System Capable of Long Polypeptide Synthesis.- Human Mitoribosome Profiling: a Re-engineered Approach Tailored to Study Mitochondrial Translation.- The ARG8m Reporter for the Study of Yeast Mitochondrial Translation.- Four Colour Super-Resolution RNA Fluorescent In Situ Hybridization and Immunocytochemistry to Visualise Mitochondrial mRNAs in Context with Mitochondrial Ribosomes.- Digital RNase Footprinting of RNA-protein Complexes and Ribosomes in Mitochondria.- Dead-seq: Discovering Synthetic Lethal Interactions from Dead Cells Genomics.