The Nuclear Receptor Superfamily

Part I: Introduction

1. Nuclear Receptors: One Big Family Iain J. McEwan

Part II: Ligand Binding and Nuclear Receptor Turnover

2. Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells Elizabeth M. Wilson

3. Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes Cherie L. Butts and Esther M. Sternberg

4. X-ray Crystallography of Agonist / Antagonist-Bound Receptors Ashley C.W. Pike

Part III: Nuclear Localization and DNA Binding

5. FRAP and FRET Methods to Study Nuclear Receptors in Living Cells Martin E. van Royen, Christoffel Dinant, Pascal Farla, J. Trapman, and Adriaan B. Houtsmuller

6. Receptor-DNA Interactions: EMSA and Footprinting Jason T. Read, Helen Cheng, Stephen C. Hendy, Colleen C. Nelson, and Paul S. Rennie

7. Chromatin Immunoprecipitation (ChIP) Methodology and Readouts Charles E. Massie and Ian G. Mills

Part IV: Nuclear Receptor-Co-regulatory Protein Interactions

8. Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors Heather A. Balsiger and Marc B. Cox

9. High Throughput Analysis of Nuclear Receptor-Cofactor Interactions Michael L. Goodson, Behnom Farboud and Martin L. Privalsky

10. Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance Derek N. Lavery

11. Using RNA Interference to Study Protein Function Carol D. Curtis and Ann M. Nardulli

12. Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Co-Activator Protein Conformation Dynamics Kate Watt and Iain J. McEwan

Part V: Pathophysiological Analysis of Nuclear Receptor Function

13. Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors Inés Pineda Torra, Julia A. Staverosky, Susan Ha, Susan K. Logan, and Michael J. Garabedian

14. Tissue-Selective Knockouts of Steroid

From the reviews:

"The primary focus is on cellular biology, biochemistry and molecular biology. ... It is written for a wide range of basic science and clinical researchers interested in transcriptional control of gene expression ... . Biomedical scientists and clinicians ... will appreciate the wealth of new information presented by the authors, all experts in the field. ... This is an outstanding contribution to the literature. ... This carefully edited collection of protocols will be valued by all those interested in steroid hormones and nuclear receptors." (Bruce A. Fenderson, Doody's Review Service, May, 2009) "This book form part of the series Methods in Molecular Biology and describes methods commonly used to investigate the structural and functional relationship of nuclear receptors, as well as the physiopathology and potential pharmacologic agents for use in therapy of these disorders. ... On consulting this book, researchers, students and professionals in the areas of biology and public health will have an opportunity to update their knowledge of experimental methods and applications of functional studies of nuclear receptors and their therapeutic implications." (Rosario D. C. Hirata, Brazilian Journal of Pharmaceutical Science, Spring, 2010)