Upstream Industrial Biotechnology, 2 Volume Set

VOLUME I: EXPRESSION SYSTEMS & PROCESS DEVELOPMENT

PREFACE xi

CONTRIBUTORS xiii

PART I INTRODUCTION 3

PART II INDUSTRIAL CELL GROWTH AND GENE EXPRESSION SYSTEMS 7

1 Animal Cells, Suspension Culture 9
John R. Birch

2 Baculovirus Expression Systems 19
Robert D. Possee, Richard B. Hitchman, and Linda A. King

3 Baculovirus Kinetics, Insect Culture 33
Leslie Chan, Steve Reid, and Lars Keld Nielsen

4 Cell Culture, Aseptic Techniques 49
John M. Davis and Kevin L. Shade

5 Cell Cycle in Bioprocesses 71
Mariam Naciri and Mohamed Al-rubeai

6 Cell Growth and Protein Expression Kinetics 85
Dhinakar S. Kompala

7 Cell Viability Measurement 97
Ning Wei and Benjamin Sommer

8 Contamination Detection in Animal Cell Culture 105
Carol Mclean and Colin Harbour

9 Culture Collections and Biological Resource Centers (BRCs) 131
David Smith

10 Culture Preservation 153
Robert L. Gherna

11 Expression and Secretion of Heterologous Proteins, Bacillus and Other Gram-Positive Bacteria 163
Boyke Bunk, Rebekka Biedendieck, Dieter Jahn, and Patricia S. Vary

12 Gene Expression in Human Cells 181
Marco A. Cacciuttolo, Gene Lee, John Chon, and John Lewis

13 Gene expression in Pichia and other methylotroph yeast 195
Koti Sreekrishna

14 Gene Expression in Recombinant Animal Cells and Transgenic Animals 213
Richard M. Twyman and Bruce Whitelaw

15 Inoculum Expansion Methods, Animal Cell Lines 297
Claudia Kloth, Glenn Maclsaac, Haile Ghebremariam, and Alahari Arunakumari

16 Insect Cell Culture 311
Someet Narang, Erik M. Whiteley, Sunyia Hussain, and Michael J. Betenbaugh

17 Kinetics of Microbial Growth 331
Nicolai S. Panikov

18 Microalgae, Mass Culture Methods 371
Emílio Molina Grima, Jose María Fernández Sevilla, and Francisco Gabriel Acién Fernández

19 Microbial Growth Measurement 399
Arthur L. Koch

20 Microbial Media Composition 413
Rosalie J. Cote

21 Microscopic Characterization of Cells 437
Erwin