Viral Vectors for Gene Therapy
Methods and Protocols
(Sprache: Englisch)
Viral Vectors for Gene Therapy: Methods and Protocols consists of 30 ch- ters detailing the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector...
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Klappentext zu „Viral Vectors for Gene Therapy “
Viral Vectors for Gene Therapy: Methods and Protocols consists of 30 ch- ters detailing the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Chapter cont- butions provide perspective in the use of viral vectors for applications in the brain and in the central nervous system. Viral Vectors for Gene Therapy: Methods and Protocols contains step-by-step methods for successful rep- cation of experimental procedures, and should prove useful for both experienced investigators and newcomers in the field, including those beginning graduate study or undergoing postdoctoral training. The "Notes" section contained in each chapter provides valuable troublesho- ing guides to help develop working protocols for your laboratory. With Viral Vectors for Gene Therapy: Methods and Protocols, it has been my intent to develop a comprehensive collection of modern molecular methods for the construction, development, and use of viral vectors for gene transfer and gene therapy. I would like to thank the many chapter authors for their contributions. They are all experts in various aspects of viral vectors, and I appreciate their efforts and hard work in developing comprehensive chapters. As editor, it has been a privilege to preview the development of Viral Vectors for Gene Therapy: Methods and Protocols, and to acquire insight into the various methodological approaches from the many different contri- tors.
Inhaltsverzeichnis zu „Viral Vectors for Gene Therapy “
Use of the Herpes Simplex Viral Genome to Construct Gene Therapy Vectors.- Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous System.- Improved HSV-1 Amplicon Packaging System Using ICP27-Deleted, Oversized HSV-1 BAC DNA.- Herpes Simplex Amplicon Vectors.- Strategies to Adapt Adenoviral Vectors for Targeted Delivery.- Use of Recombinant Adenovirus for Gene Transfer into the Rat Brain.- Generation of Adenovirus Vectors Devoid of All Viral Genes by Recombination Between Inverted Repeats.- Packaging Cell Lines for Generating Replication-Defective and Gutted Adenoviral Vectors.- Improving the Transcriptional Regulation of Genes Delivered by Adenovirus Vectors.- Targeted Integration by Adeno-Associated Virus.- Development and Optimization of Adeno-Associated Virus Vector Transfer into the Central Nervous System.- A Method for Helper Virus-Free Production of Adeno-Associated Virus Vectors.- Novel Tools for Production and Purification of Recombinant Adeno-Associated Viral Vectors.- Recombinant Adeno-Associated Viral Vector Types 4 and 5.- Trans-Splicing Vectors Expand the Packaging Limits of Adeno-Associated Virus for Gene Therapy Applications.- Generation of Retroviral Packaging and Producer Cell Lines for Large-Scale Vector Production with Improved Safety and Titer.- An Ecdysone-Inducible Expression System for Use with Retroviruses.- In Vivo Infection of Mice by Replication-Competent MLV-Based Retroviral Vectors.- Development of Simian Retroviral Vectors for Gene Delivery.- Self-Inactivating Lentiviral Vectors and a Sensitive Cre-loxP Reporter System.- Lentiviral Vectors for Gene Transfer to the Central Nervous System.- A Highly Efficient Gene Delivery System Derived from Feline Immunodeficiency Virus (FIV).- A Multigene Lentiviral Vector System Based on Differential Splicing.- Production of Trans-Lentiviral Vector with Predictable Safety.- Human Immunodeficiency Virus Type 1-Based Vectors for Gene Delivery to Human
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Hematopoietic Stem Cells.- Semliki Forest Viral Vectors for Gene Transfer.- Semliki Forest Virus (SFV) Vectors in Neurobiology and Gene Therapy.- Semliki Forest Virus Vectors for Large-Scale Production of Recombinant Proteins.- Development of Foamy Virus Vectors.- Poxviral/Retroviral Chimeric Vectors Allow Cytoplasmic Production of Transducing Defective Retroviral Particles.
... weniger
Bibliographische Angaben
- 2010, XVI, 592 Seiten, Maße: 15,2 x 22,9 cm, Kartoniert (TB), Englisch
- Herausgegeben: Curtis A. Machida
- Verlag: Springer, Berlin
- ISBN-10: 1617373087
- ISBN-13: 9781617373084
Sprache:
Englisch
Pressezitat
"This text would be very good for someone who can appreciate the many and varied practical aspects of performing a gene therapy study. I would certainly recommend it to anyone I knew working in the gene therapy field as a book to compare and contrast practical approaches and requirements for various viral vectors." - Pharmaceutical Research
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