Yeast Protocols

Basic Investigations in Saccharomyces cerevisiae.- Isolation of Yeast Nucleic Acids.- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry.- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe.- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry.- Yeast Two Hybrid Liquid Screening.- Targeted Mutagenesis of a Specific Gene in Yeast.- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae.- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy.- Assays for Autophagy II: Mitochondrial Autophagy.- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction.- Yeast Survival and Growth Assays.- Spontaneous Mutagenesis Assay.- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene.- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe.- Replicative and Chronological Life Span Assays.- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae.- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae.- Ligand Engineering Using Yeast Surface Display.- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay.