Chemical Determinations (PDF)

Chemical Determinations is a collection of papers that discusses chemical methods used in hormone investigation. One paper explains the physicochemical procedures used to separate estrogens from interfering material which leads to the possible determination of small amounts of hormones in biological fluids. One approach is through simpler methods for routine use, designed principally for the determination of estrogens in urine and in plasma. Another way is through more elaborate methods, designed to establish the presence and identity of various estrogens in small numbers of biological samples. Another paper explains the methods for the analysis of 17-ketosteroids of blood and urine, and a method for the assay of testosterone in plasma which correlates with the state of virilism of an individual. One paper describes the techniques utilized for extraction, preliminary purification, isolation, and quantitative estimation of progesterone. Another paper reviews the occurrence of pregnanetriolone (an inactive urinary metabolite), the significance of its estimation as an aid in differential diagnosis, the methods of estimation, as well as the metabolic pathways leading to the excretion of pregnanetriolone and related steroids. The collection is suitable for researchers, scientists, medical practitioners, or academicians involved in endocrinology, metabolism, and bio-research.