Experimental Approaches for the Investigation of Innate Immunity (ePub)
The Human Innate Immunity Handbook
(Sprache: Englisch)
The recent explosion of information in innate immune pathways for recognition, effect or responses, and genetic regulation has given impetus to investigations into analogous pathways in the human immune response, which in turn has produced attendant...
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The recent explosion of information in innate immune pathways for recognition, effect or responses, and genetic regulation has given impetus to investigations into analogous pathways in the human immune response, which in turn has produced attendant insights into both normal physiology and immunopathology. This volume presents a compendium of methods and protocols for the investigation of human innate immunity with application to the study of normal immune function, immunosenescence, autoimmunity and infectious diseases. Among the topics covered are quantitative flow cytometry for Toll-like receptor expression and function; multidimensional single cell mass cytometry (CyTOF) in complex immune interactions and tumor immunity; imaging techniques such as Imagestream high resolution microscopy coupled to flow cytometry, immune cell infiltration of organotypic, biomimetic organs; high-throughput single cell secretion profiling; multiplexed transcriptomic profiling; microsatellite and microRNA methodologies, RNA interference; and the latest bioinformatics and biostatistical methodologies, including in-depth statistical modeling, genetic mapping, and systems approaches.
Autoren-Porträt
Ruth R Montgomery received her PhD from Rockefeller University where she studied with Zanvil Cohn and Carl Nathan. After postdoctoral work on macrophage endocytosis with Ira Mellman, she remained at Yale to investigate innate immunity. In translational studies using primary human cells, the Montgomery lab investigates effects of aging on innate immunity, including dysregulation of Toll-like receptors, and innate immune responses that play a role in the pathogenesis of Hepatitis C and West Nile viruses.Richard Bucala, received his MD and PhD degrees from the combined Rockefeller-Cornell University program. After a visiting fellowship at the Institut Pasteur and residency training at the Brigham & Women's Hospital and Harvard Medical School, he returned to Rockefeller for a junior faculty position and subspecialty training in rheumatology at the Hospital for Special Surgery. He is currently Professor of Medicine, Pathology, and Epidemiology and Public Health at Yale. His research interests focus on the interplay of host determinants for autoimmunity and infectious diseases susceptibility.
Ruth R Montgomery received her PhD from Rockefeller University where she studied with Zanvil Cohn and Carl Nathan. After postdoctoral work on macrophage endocytosis with Ira Mellman, she remained at Yale to investigate innate immunity. In translational studies using primary human cells, the Montgomery lab investigates effects of aging on innate immunity, including dysregulation of Toll-like receptors, and innate immune responses that play a role in the pathogenesis of Hepatitis C and West Nile viruses.
Richard Bucala, received his MD and PhD degrees from the combined Rockefeller-Cornell University program. After a visiting fellowship at the Institut Pasteur and residency training at the Brigham & Women's Hospital and Harvard Medical School, he returned to Rockefeller for a junior faculty position and subspecialty training in
... mehr
rheumatology at the Hospital for Special Surgery. He is currently Professor of Medicine, Pathology, and Epidemiology and Public Health at Yale. His research interests focus on the interplay of host determinants for autoimmunity and infectious diseases susceptibility.
... weniger
Bibliographische Angaben
- 2016, Englisch
- Herausgegeben: Richard Bucala, Ruth R Montgomery
- ISBN-10: 9814678732
- ISBN-13: 9789814678735
- Erscheinungsdatum: 15.01.2016
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- Dateiformat: ePub
- Größe: 2.06 MB
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Sprache:
Englisch
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