Practical Methods for Biocatalysis and Biotransformations (PDF)
(Sprache: Englisch)
Biocatalysts are increasingly used by chemists engaged in fine
chemical synthesis within both industry and academia. Today, there
exists a huge choice of high-tech enzymes and whole cell
biocatalysts, which add enormously to the repertoire of...
chemical synthesis within both industry and academia. Today, there
exists a huge choice of high-tech enzymes and whole cell
biocatalysts, which add enormously to the repertoire of...
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Biocatalysts are increasingly used by chemists engaged in fine
chemical synthesis within both industry and academia. Today, there
exists a huge choice of high-tech enzymes and whole cell
biocatalysts, which add enormously to the repertoire of synthetic
possibilities.
Practical Methods for Biocatalysis and Biotransformations
is a "how-to" guide focussing on commercially available
enzymes and strains of microorganisms that are readily obtained
from culture collections. The source of starting materials and
reagents, hints, tips and safety advice (where appropriate) are
given to ensure, as far as possible, that the procedures are
reproducible. Comparisons to alternative methodology are given and
relevant references to the primary literature are cited. Contents
include:
* Introduction to the application of biocatalysts in the pharma
industry
* introduction to molecular biological techniques for biocatalyst
development
* Lipase and protease methods including enzyme supports and
dynamic kinetic resolutions
* Nitrile hydrolases and hydratases
* Alcohol dehydrogenases and co-factor recycling
* Epoxide hydrolases
* Amino acid synthesis
* Whole cell processes
* Other biocatalysts, including monoamineoxidases,
aminotransterases, and lyases
Practical Methods for Biocatalysis and Biotransformations
is an essential text offering validated biocatalyst methods for
researchers and postgraduate students in industry and academia.
chemical synthesis within both industry and academia. Today, there
exists a huge choice of high-tech enzymes and whole cell
biocatalysts, which add enormously to the repertoire of synthetic
possibilities.
Practical Methods for Biocatalysis and Biotransformations
is a "how-to" guide focussing on commercially available
enzymes and strains of microorganisms that are readily obtained
from culture collections. The source of starting materials and
reagents, hints, tips and safety advice (where appropriate) are
given to ensure, as far as possible, that the procedures are
reproducible. Comparisons to alternative methodology are given and
relevant references to the primary literature are cited. Contents
include:
* Introduction to the application of biocatalysts in the pharma
industry
* introduction to molecular biological techniques for biocatalyst
development
* Lipase and protease methods including enzyme supports and
dynamic kinetic resolutions
* Nitrile hydrolases and hydratases
* Alcohol dehydrogenases and co-factor recycling
* Epoxide hydrolases
* Amino acid synthesis
* Whole cell processes
* Other biocatalysts, including monoamineoxidases,
aminotransterases, and lyases
Practical Methods for Biocatalysis and Biotransformations
is an essential text offering validated biocatalyst methods for
researchers and postgraduate students in industry and academia.
Inhaltsverzeichnis zu „Practical Methods for Biocatalysis and Biotransformations (PDF)“
Preface. Abbreviations. List of Contributors. 1 Biotransformations in Small-molecule Pharmaceutical Development (Joseph P. Adams, Andrew J. Collis, Richard K. Henderson and Peter W. Sutton). 2 Biocatalyst Identification and Scale-up: Molecular biology for Chemists (Kathleen H. McClean). 3 Kinetic Resolutions Using Biotransformations. 4 Dynamic Kinetic Resolution for the Synthesis of Esters, Amides and Acids Using Lipases. 5 Enzymatic Electivity in Synthetic Methods. 6 Aldolase Enzymes for Complex Synthesis. 7 Enzymatic Synthesis of Glycosides and Glucuronides. 8 Synthesis of Cyanohydrins Using Hydroxynitrile Lyases. 9 Synthesis of Chiral sec-Alcohols by Ketone Reduction. 10 Reduction of Functional Groups. 11 Enzymatic Oxidation Chemistry. 12 Whole-cell Oxidations and Dehalogenations. Index.
Autoren-Porträt
John WhittallCoEBio3, Manchester Interdisciplinary Biocentre, Manchester
University, UK
Peter W Sutton
GlaxoSmithKline Research and Development Limited, UK
Bibliographische Angaben
- 2009, 1. Auflage, 432 Seiten, Englisch
- Herausgegeben: John Whittall, Peter W. Sutton
- Verlag: John Wiley & Sons
- ISBN-10: 0470748591
- ISBN-13: 9780470748596
- Erscheinungsdatum: 25.11.2009
Abhängig von Bildschirmgröße und eingestellter Schriftgröße kann die Seitenzahl auf Ihrem Lesegerät variieren.
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- Größe: 9.59 MB
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