Tag-based Next Generation Sequencing (ePub)

TAG-BASED NUCLEIC ACID ANALYSIS DeepSuperSAGE: High-throughput transcriptome sequencing with now- and next-generation sequencing technologies (Hideo Matsumura, Carlos Molina, Detlev H. Krüger, Ryohei Terauchi and Günter Kahl) DeepCAGE: Genome-wide mapping of transcription start sites (Matthias Harbers, Mitch Dushay and Piero Carninci) Definition of promotome-transcriptome architecture using CAGEscan (Nicolas Bertin, Charles Plessy, Piero Carninci and Matthias Harbers) RACE: New applications of an old method to connect exons (Charles Plessy) RNA-PET: Full-Length transcript analysis using 5? and 3? paired-end-tag next generation sequencing (Xiaoan Ruan and Yijun Ruan) Stranded RNA-Seq: Strand specific shotgun sequencing of RNA (Alistair R.R. Forrest) Differential RNA sequencing (dRNA-seq): Deep-sequencing based analysis of primary transcriptomes (Anne Borries, Jörg Vogel and Cynthia M. Sharma) Identification and expression profiling of small RNA populations using high-throughput sequencing (Javier Armisen, W. Robert Shaw and Eric A. Miska) Genome-wide mapping of protein-DNA interactions by ChIP-seq (Joshua W.K. Ho, Artyom A. Alekseyenko, Mitzi I. Kuroda and Peter J. Park) Analysis of protein-RNA interactions with single-nucleotide resolution using iCLIP and next generation sequencing (Julian König, Nicholas J. McGlincy and Jernej Ule) Massively parallel tag sequencing unveils the complexity of marine protistan communities in oxygen-depleted habitats (Virginia Edgcomb and Thorsten Stoeck) Chromatin interaction analysis using paired-end-tag sequencing (ChIA-PET) (Xiaoan Ruan and Yijun Ruan) Tag-seq: Next-generation tag sequencing for gene expression profiling (Sorana Morrissy, Yongjun Zhao, Allen Delaney, Jennifer Asano, Noreen Dhalla, Irene Li, Helen McDonald, Pawan Pandoh, Anna-Liisa Prabhu, Angela Tam, Martin Hirst and Marco Marra) Isolation of active regulatory elements from eukaryotic chromatin using FAIRE (Formaldehyde Assisted Isolation of Regulatory Elements)

Matthias Harbers works in the Japanese biotechnology industry and holds a position as Senior Visiting Scientist at the RIKEN Omics Science Center in Yokohama, Japan. After gaining his PhD at the University Hamburg (Germany), he worked at the Karolinska Institute in Stockholm (Sweden) and the Institut de Génétique et de Biologie Moléculaire et Cellulaire in Strasbourg (France).
Main research areas are:
-Transcriptome analysis
-Preparation of genomic resources
-Biomarker discovery
Matthias Harbers contributed to advanced scientific publications in high-ranking journals such as Science, PNAS, Nature Genetics, Nature Methods, Journal of Biological Chemistry, Nucleic Acids Research, Genome Research, and Genes and Development among others.

Günter Kahl is Professor for Plant Molecular Biology at Frankfurt University, Germany. After his PhD in plant biochemistry, he spent two years with Joe Varner, MSU, East Lansing, and James Bonner, CalTech, Pasadena, USA.
Main research areas:
-Sequencing and analysis of fungal, plant and animal genomes
-Transcriptome analysis in pro- and eukaryotic organisms
-Technology development
Günter Kahl cooperates with a series of research institutions throughout Europe, in Japan, the USA, Israel, India, and Latin America. He served in expert missions for IAEA, FAO, and UNESCO in many countries, and is presently Chief Scientific Officer in the SME GenXPro GmbH at the Frankfurt Innovation Center Biotechnology.

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