CRISPR-Cas Enzymes

1. Predicting and visualizing features of CRISPR-Cas systems Matthew A. Nethery and Rodolphe Barrangou 2. Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems Yibei Xiao and Ailong Ke 3. Sortase-mediated fluorescent labeling of CRISPR complexes Kaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein 4. Fluorescence-based methods for measuring target interference by CRISPR-Cas systems Phong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital 5. Probing Cascade complex composition and stability using native mass spectrometry techniques Angela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner 6. High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP Joseph T. Wade 7. Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance Bartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau 8. In vitro assembly of thermostable Csm complex in CRISPR-Cas type III/A system Kwang-Hyun Park, Yan An and Eui-Jeon Woo 9. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems Christophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White 10. A pipeline for characterization of novel Cas9 orthologs Tautvydas Karvelis, Joshua K. Young and Virginijus Siksnys 11. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek 12. Directed evolution studies of a thermophilic Type II-C Cas9 Travis H. Hand, Anuska Das and Hong Li 13. Kinetic characterization of Cas9 enzymes Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W.Taylor and