DNA Repair Enzymes: Structure, Biophysics, and Mechanism

1. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes Scott D. Gradia, Justin P. Ishida, Miaw-Sheue Tsai, Chris Jeans, John A. Tainer and Jill O. Fuss 2. Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes David M. Rees, Oliver Willhoft, Chia-Liang Lin, Rohan Bythell-Douglas and Dale B. Wigley 3. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR Matthew K. Thompson, Aaron C. Ehlinger and Walter J. Chazin 4. Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair Peter Friedhoff, Laura Manelyte, Luis Giron-Monzon, Ines Winkler, Flora Groothuizen and Titia K. Sixma 5. Expression and Structural Analyses of Human DNA Polymerase ? (POLQ) Andrew W. Malaby, Sara K. Martin, Richard D. Wood and Sylvie Doublié 6. Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes Malgorzata Figiel and Marcin Nowotny 7. DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage Dimitri Y. Chirgadze, David B. Ascher, Tom L. Blundell and Bancinyane L. Sibanda 8. Single-Particle Electron Microscopy Analysis of DNA Repair Complexes Marta Sawicka, Ricardo Aramayo, Rafael Ayala, Robert Glyde and Xiaodong Zhang 9. Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes that Carry Out DNA Repair Sharonda LeBlanc, Hunter Wilkins, Zimeng Li, Parminder Kaur, Hong Wang and Dorothy A. Erie 10. Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time Muwen Kong, Emily C. Beckwitt, Luke Springall, Neil M. Kad and Bennett Van Houten 11. Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination Michael M. Soniat, Logan R. Myler, Jeffrey M. Schaub, Yoori Kim, Ignacio F. Gallardo and Ilya J. Finkelstein 12. Detection of Reaction