Conditional Mutagenesis: An Approach to Disease Models

Part 1: Tools for Conditional Mutagenesis.- Conditional Somatic Mutagenesis in the Mouse Using Site-Specific Recombinases. Cre/loxP-mediated Chromosome Engineering of the Mouse Genome. Tetracycline-Controlled Genetic Switches Novel Gene Switches. Improved Embryonic Stem Cell Technologies. Gene Trap Mutagenesis. RNA Interference in Mice. Viral Vectors: A Wide Range of Choices and High Levels of Service. Conditional mutagenesis by cell permeable proteins: Potential, Limitations and Prospects.- Part 2: Examples of Conditional Disease Models.- Analysis of Mouse Development with Conditional Mutagenesis. Conditional Mouse Models of Cancer. Conditional Mutagenesis Reveal Immunological Functions of Widely Expressed Genes: Activation Thresholds, Homeostatic Mechanisms and Disease Models. Conditional Transgenesis and Recombination to Study the Molecular Mechanisms of Brain Plasticity and Memory. A Novel Conditional Knockout Strategy Applied to Serotonin Receptors. Conditional Mouse Models for Friedreich Ataxia, A Neurodegenerative Disorders Associating Cardiomyopathy. Animal Models in Cardiovascular Diseases: New Insights from Conditional Models. Conditional Animal Models for the Study of Lipid Metabolism and Lipid Disorders. Conditional Mouse Models to Study Developmental and Pathological Gene Function in Muscle. Analysis of Calcium Channels by Conditional Mutagenesis. Conditional Mutagenesis of G-protein Coupled Receptors and G-proteins. Contribution of Targeted Conditional Somatic Mutagenesis to Deciphering Retinoid X receptor (RXR) Functions and to Generating Mouse Models of Human Diseases.