A Beginner's Guide to Microarrays
(Sprache: Englisch)
A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and...
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A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides,
-Designing oligonucleotide probes,
-Constructing arrays for the detection and quantification of different bacterial species,
-Preparing spotting solutions,
-Troubleshooting spotting problems,
-Setting up and running a core facility,
-Normalizing background signal and controlling for systematic variance,
-Designing experiments for maximum effect,
-Analyzing data with statistical procedures,
-Clustering data with machine-learning protocols.
Inhaltsverzeichnis zu „A Beginner's Guide to Microarrays “
- 1: Slide Coating And DNA Immobilization Chemistries- 2: Diagnostic Oligonucleotide Microarrays For Microbiology
- 3: Printing Technologies And Microarray Manufacturing Techniques: Making The Perfect Microarray
- 4: Arrays For The Masses - Setting Up A Microarray Core Facility
- 5: Microarray Data Normalization: The Art And Science Of Overcoming Technical Variance To Maximize The Detection Of Biologic Variance
- 6: Experimental Design And Data Analysis
- 7: Microarray Experiment Design And Statistical Analysis
- 8: Strategies For Clustering, Classifying, Integrating, Standardizing And Visualizing Microarray Gene Expression Data
Bibliographische Angaben
- 2003, 347 Seiten, Maße: 23,5 cm, Gebunden, Englisch
- Ed. by Eric M. Blalock
- Verlag: Springer Netherlands
- ISBN-10: 1402074727
- ISBN-13: 9781402074721
Sprache:
Englisch
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