Bioanalytics for Beginners

reactions are in the time progress      3.8 Unit and Katal: the measurement of enzyme activity      3.9 Getting started: optical enzyme tests      3:10 Dry chemistry: from the litmus test paper for glucose-testing      3.11 Inhibition of enzyme reactions      3.12 Bird dead or...: the exact measurement of enzyme inhibitors      3.13 Isoenzymes      3.14 Enzyme-activity testsCHAPTER 4 Bio-affinity I: antibodies and immune tests 75      4.1 Is War vaccination successful? The ring test      4.2 How antigens and antibodies react with Haptene      4.3 Blood is a very special juice: determination of blood groups      4.4 Soluble + soluble = insoluble: immune precipitation      4.5 Diffusion combined with electrophoresis: immune electrophoresis      4.6 Useful protein blurs: Western blotting      4.7 Nephelometry: to fish in the dark with success      4.8 Immuno assays: "The best [perfect] is the enemy of the good"      4.9 Thyroid tests with the Radio immuno assay      4.10 Immunology with the power of enzymes: ELISA      4.11 Indirect ELISA: detection of antibodies against HIV and the Dot test      4.12 Rapid immuno-tests: a baby - yes or no?      4.13 The rapid detection of a death angel: HIV tests      4.14 Quick help with heart attacks      4.15 A worldwide trend: Point of care (POC) tests      4.16 Drugs and their abuse     4.17 Immunological drug tests      4.18 How to evaluate a laboratory test: sample HIV testing      4.19 How to test tests: ROC-curves CHAPTER 5 Bio-affinity II: biological receptors - nature as unsurpassed Bioanalysis lab worker      5.1 The fantastic dog nose: A million-times better than ours!      5.2 Our human meaning     5.3 Smell: olfactory detection      5.4 How does a receptor work?      5.5 Electronic nose: Combined polymers versus real receptors      5.6 Taste: gustatory detection      5.7 Visual detection      5.8 The evolution of the eye     5.9 Processes in the retina      5.10 Seeing colours      5.11 Listen: acoustic detection      5.12 Molecular mechanisms of hearing      5.13 Touch: tactile detection      5.14 Are there any other sensory systems?CHAPTER 6 DNA, RNA, and their amplification      6.1 DNA: the double helix      6.2 Rools for DNA analysis: DNA polymerase      6.3 DNA to RNA: RNA polymerase      6.4 In short: the DNA code     6.5 Structural genes, exons and introns      6.6 Plasmid as a DNA cuckoo     6.7 DNA scissors and glue: restriction endonucleases and ligases      6.8 Inverted world: RNA into DNA by reverse transcriptase      6.9 How to gain nucleic acids     6.10 Experiment! DNA isolated from zucchini in the kitchen      6.11 Determination of optic concentration of nucleic acids      6.12 DNA probes to detect DNA     6.13 How to analyze DNA: gel electrophoresis separates DNA fragments according to their size      6.14 Life and death: genetic fingerprints to detect paternity or murder      6.15 DNA markers: tandems and "snippets"      6.16 The polymerase chain reaction: DNA copier     6.17 Reverse transcriptase-PCR for the detection of RNA viruses      6.18 The real-time PCR (RTQ-PCR) quantifies PCR products      6:19 How genes are sequenced      6.20 Southern blotting      6:21 Automatic DNA sequencing      6.22 FISH: chromosomal location and number of gene copies CHAPTER 7 Biosensors      7.1 Enzyme tests for millions of diabetics     7.2 Diabetes mellitus - what to do?      7.3 Glucose biosensors: highly effective combinations of biomolecules and sensors      7.4 Glucose-test yourself!     7.5 Environmental control by microbial respirometry: the BOD5 test      7.6 Cell sensors measure the sewage pollution in five minutes      7.7 Molecular dog hunting: Piezoelectric sensors      7.8 Mirage, optical sensors, and BIAcore      7.9 Real-time measurements with SPR      7.10 How can one detect deadly substances with antibodies?      7.11 How does one generate a signal?     7.12 How do the antibodies of immunosensors remain functional?      7.13 biosensors with immobilized antibodies      7.14 Biochips: Targeted molecular fishing in the dark      7.15 How does a DNA chip work?      7.16 How to figure out morbidity dates and diagnose viruses      7.17 DNA chips for cytochrome P450      7.18 DANN chips for green genetic engineering, ecology, and forensics      7.19 Protein chips CHAPTER 8 Pharmaceuticals on the bench      8.1 Bioanalysis conquers drug labs      8.2 From green to red-gold vein      8.3 High-throughput drug screening for active drug candidates      8.4 New HPLC screening techniques      8.5 RP-HPLC coupled with nuclear resonance spectroscopy (NMR)      8.6 Matrix-based Laser Desorption Ionisation Mass Spectrometry (MALDI-MS)      8.7 Electrospray Ionisation Mass Spectrometry (ESI-MS)      8.8 New in vitro and in vivo tests by genetically modified organisms (GMOs): finally, the answers-or more questions?      8.9 Consideration of recombinant pharmaceuticals INSERT: BIOANALYSIS - an independent science: a timetable on the first and last book pages GLOSSARYCREDITSINDEX