Biological Applications of Microfluidics

Preface. Contributors. 1 Microfluidics. 1.1 Microfluidics. PART I CELL ANALYSIS ON MICROFLUIDIC DEVICES. 2 Using Microfluidics to Understand and Control the Cellular Microenvironment. 2.1 Introduction: Engineering the Microenvironment. 2.2 The Chemical Microenvironment. 2.3 The Mechanical Microenvironment. 2.4 Conclusion. 3 Microfabricated Devices for Cell Sorting. 3.1 Introduction. 3.2 Microfabricated Formats for Cell Sorting. 3.3 Outlook for the Future. 4 Advanced Microfluidic Tools for Single Cell Manipulation and Analysis. 4.1 Introduction. 4.2 Fluidic Control. 4.3 Temperature Control. 4.4 Cell Manipulation. 4.5 Detection. 4.6 Integration. 4.7 Conclusions. 5 Engineering Cellular Microenvironments with Microfluidics. 5.1 Introduction. 5.2 Microfluidic Cultures can Simulate in vivo Microenvironments. 5.3 Other Useful Capabilities of Microfluidic Cell Culture Devices. 5.4 Microfluidic Devices Useful for Cell Applications Other than Culture. 5.5 Future Prospects for Biological Studies in Microfluidic Bioreactors. 6 Microfluidic Culture Platforms for Stem Cell and Neuroscience Research. 6.1 Introduction. 6.2 Applications for Stem Cell Research. 6.3 Applications for Neuroscience Research. 6.4 Summary and Future Directions. PART II ENZYMATIC AND NONENZYMATIC REACTIONS ON MICROCHIPS. 7 Microfluidics for Studying Enzyme Inhibition. 7.1 Enzyme Assays and Inhibition. 7.2 Microfluidic Assays for Enzymes and Enzyme Inhibition. 7.3 Enzyme Inhibition Studies in Microfluidic Devices: Specific Studies. 8 Chemical Synthesis within Continuous Flow Microreactors. 8.1 Introduction. 8.2 Advantages of Performing Chemical Synthesis in Microreactors. 8.3 Chemical Synthesis in Microreactors. 8.4 Large Scale Manufacture Using Microreactors. 8.5 Conclusions. 9 Microfluidic Reactors for Sequential and Parallel Reactions. 9.1 Introduction. 9.2 Sequential Reactions in Microfluidic Devices. 9.3 Parallel Reactions in Microfluidic Devices. 9.4 Conclusions. 10 Gene Isolation, Gene Transformation,

Frank A. Gomez, PhD, is the Director of the CSULA-Caltech Partnership for Research and Education in Materials (PREM) Collaborative. He is a Professor in the Department of Chemistry and Biochemistry at California State University, Los Angeles, and a Visiting Research Associate at the California Institute of Technology.

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