Biophysical Tools for Biologists.Vol.2

Section 1. Fluorescence Methods
1) Photoactivation and Photobleaching Techniques for Analysis of Organelle Biogenesis in vivo
2) Analysis of the Dynamics of Living Cells by Fluorescence Correlation Spectroscopy
3) Molecular Sensors Based on Fluorescence Resonance Energy Transfer to Visualize Cellular Dynamics 4) Real-Time Fluorescence of Protein Folding in vivo
5) Microfluidic Glucose Stimulation of Ca+2 Oscillations in Pancreatic Islets

Section 2. Microscopic Methods
6) Introduction to Optical Sectioning: Confocal, Deconvolution, and Two-Photon
7) Use of Electron Tomography to Elucidate Sub-Cellular Structure and Function
8) Proteomics of Macromolecular Complexes by Cellular Cryo-Electron Tomography
9) Total Internal Reflectance Microscopy (TIRF)
10) Atomic Force Microscopy of Living Cells
11) Real-Time Kinetics of Gene Activity in Individual Bacteria
12) Measurement of Cytoskeletal Proteins Globally and Locally in vivo
13) Infrared and Raman Microscopy in Cell Biology
14) Imaging Fluorescent Mice in vivo by Confocal Microscopy
15) Nanoscale Imaging of Intracellular Fluorescent Proteins: Breaking the Diffraction Barrier

Section 3. Methods at the In Vitro/In Vivo Interface
16) Analysis of Protein Posttranslational Modification by Mass Spectrometry
17) Imaging Mass Spectrometry
18) Wet EM Using Quantum Dots
19) Single Cell Capillary Electrophoresis

Section 4. Methods for Diffusion, Viscosity, Force and Displacement
20) Single-Molecule Force Spectroscopy in Living Cells
21) Magnetic Bead Force Applications
22) Measurement of Membrane-Cytoskeleton Adhesion Using Laser Optical Tweezers
23) Cellular Rheological Measurements in vivo
24) Physical Behavior of Cytoskeletal Networks in vitro and in vivo
25) Force Regulation of Microtubule Dynamics in Fission Yeast

Section 5. Techniques for Protein Activity, Protein-Protein and Protein-RNA Interactions
26) Quantifying Protein Activity Using FRET and FLIM Microscopy
27)

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.