Plant Protoplasts and Genetic Engineering I

Section I Isolation Culture and Preservation of Protoplasts.- I.1 Recent Advances in the Isolation and Culture of Protoplasts and Their Implications in Crop Improvement.- I.2 Enzymes for the Isolation of Protoplasts.- I.3 Viability of Plant Protoplasts.- I.4 Agarose Plating of Protoplasts and Its Applications.- I.5 Immobilization of Plant Protoplasts.- I.6 Cryopreservation of Plant Protoplasts.- Section II Regeneration of Plants from Protoplasts.- II.1 Regeneration of Plants from Rice Protoplasts.- II.2 Isolation and Culture of Potato Protoplasts.- II.3 Isolation and Culture of Citrus Protoplasts.- II.4 Regeneration of Plants from Linum Protoplasts.- II.5 Isolation and Culture of Glycine Protoplasts.- II.6 Regeneration of Plants from Cabbage (Brassica oleracea var. capitata) Protoplasts.- II.7 Regeneration of Plants from Chicory (Chicorium intybus L.) Protoplasts.- II.8 Regeneration of Plants from Protoplasts of Lettuce and its Wild Species.- II.9 Regeneration of Plants from Protoplasts of Butterbur (Petasites japonicus, Fr. Schmidt).- II.10 Isolation and Culture of Orchid Protoplasts.- II.11 Regeneration of Plants from Protoplasts of Salpiglossis.- II.12 Isolation and Culture of Protoplasts of Forest Tree Species.- Section III Protoplast Fusion and Somatic Hybridization.- III.1 Fusion of Plant Protoplasts - Techniques.- III.2 Ultrastructural Studies of Plant Protoplast Fusion.- III.3 Symmetric Versus Asymmetric Fusion Combinations in Higher Plants.- III.4 Somatic Hybridization in Tomato.- III.5 Lycopersicon and Petunia Somatic Hybrids.- III.6 Somatic Hybridization in Hyoscyamus and Nicotiana.- III.7 Medicago Protoplasts: Fusion, Culture and Plant Regeneration.- III.8 Somatic Hybridization Between Moricandia and Brassica.- III.9 Somatic Hybridization in Trifolium.-III.10 Somatic Hybridization in Rice × Soybean.