Cellular and Molecular Methods in Neuroscience Research

Analyses of Intracellular Signal Transduction Pathways in CNS Cells Confocal and Electron Microscopic Tracking of Internalized Neuropeptide/Receptor Complexes Transfection Methods for Neurons in Primary Culture Polyethylenimine: a Versatile Cationic Polymer for Plasmid-based Gene Delivery in the CNS Trasfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK293 Cells Neuronal Transfection Using Particle-Mediated Gene Transfer Analysis of Gene Expression in Genetically Labeled Single Cells Immunocytochemistry and In Situ Hybridization: Interest of their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons In Situ Reverse Transcriptase PCR for Detection of mRNA in the CNS Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity Combined electrophysiological and morphological analysis of CNS neurons Tract-Tracing Methods at the Light Microscopy Level Tract-tracing Methods at theUltrastructural Level In vivo Analysis of Cell Proliferation and Apoptosis in the CNS Confocal Imaging of Nerve Cells and their Connections Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices